CYPs hydroxylate DHA to 14(R)-HDHA

Stable Identifier
R-HSA-9027043
Type
Reaction [transition]
Species
Homo sapiens
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Using human leukocytes and platelets, Hong et al. found that incubating docosahexaenoic acid (DHA) with a mixture of human cytochrome P450 enzymes (CYP1A2, 2C8, 2C9, 2D6, 2E1 and 3A4) formed 14(R)-hydroxy-docsosahexaenoic acid (14(R)-HDHA), the precursor to maresin-like mediator 2 (MaR-L2) (Hong et al. 2014). There is no data to indicate which one or ones of these CYPs mediate this event under physiological conditions. CYP inhibition diminishes the formation of 14(R)-HDHA, confirming CYP determines 14(R)-hydroxylation (Hong et al. 2014). The CYP mixture also formed 22-hydroxy-docosahexaenoic acid (22-HDHA) by ω-oxidation (not shown here). Although the CYP mixture also catalysed the formation of 14(S)-HDHA, the amount formed is minor compared to that formed by 12-lipoxygenase (Hong et al. 2014).

Literature References
PubMed ID Title Journal Year
25200603 Maresin-like lipid mediators are produced by leukocytes and platelets and rescue reparative function of diabetes-impaired macrophages

Hong, S, Lu, Y, Tian, H, Alapure, BV, Wang, Q, Bunnell, BA, Laborde, JM

Chem. Biol. 2014
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Participant Of
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Catalyst Activity
Title
monooxygenase activity of CYP1A2,2C8,2C9,2D6,2E1,3A4 [endoplasmic reticulum membrane]
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Orthologous Events
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