RHOU binds effectors at the plasma membrane

Stable Identifier
Homo sapiens
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In its active GTP bound form, RHOU binds the following effectors:
ARHGAP30 (Naji et al. 2011; protein with GAP activity)
ARHGAP31 (Naji et al. 2011; protein with GAP activity)
GRB2 (Zhang et al. 2011; Bagci et al. 2020: weak binding)
ITSN2 (Gubar et al. 2020)
NCK2 (Saras et al. 2004; Bagci et al. 2020)
PAK1 (Tao et al. 2001; Shutes et al. 2004; Saras et al. 2004; Bagci et al. 2020)
PAK4 (Dart et al. 2015)
PAR6 (Brady et al. 2009)
PIK3R1 (Chuang et al. 2007; Bagci et al. 2020; protein with GAP activity)
PTK2B (Ruusala and Aspenström 2008)

The following candidate RHOU effectors were identified in the high throughput screen by Bagci et al. 2020:
ARHGEF6 (Bagci et al. 2020)
ARHGEF7 (Bagci et al. 2020)
CDC42 (Bagci et al. 2020)
CLTC (Bagci et al. 2020)
DEPDC1B (Bagci et al. 2020; protein with GAP activity)
DLG5 (Bagci et al. 2020)
DST (Bagci et al. 2020)
EPHA2 (Bagci et al. 2020)
GIT1 (Bagci et al. 2020)
GIT2 (Bagci et al. 2020)
HGS (Bagci et al. 2020)
IQGAP1 (Bagci et al. 2020)
MYO6 (Bagci et al. 2020)
NCK1 (Bagci et al. 2020)
PAK2 (Bagci et al. 2020)
PAK3 (Bagci et al. 2020)
PIK3R2 (Bagci et al. 2020; protein with GAP activity)
PEAK1 (Bagci et al. 2020)
SPTAN1 (Bagci et al. 2020)
SPTBN1 (Bagci et al. 2020)
SRGAP2 (Bagci et al. 2020; protein with GAP activity)
STAM (Bagci et al. 2020)
STAM2 (Bagci et al. 2020)
TXNL1 (Bagci et al. 2020)
USP9X (Bagci et al. 2020)
VANGL1 (Bagci et al. 2020)
WDR6 (Bagci et al. 2020)
WWP2 (Bagci et al. 2020)

RHOU does not bind WASL, a component of the WIP WASP complex (Bagci et al. 2020) and also does not bind:
CCP110 (Bagci et al. 2020)
CEP97 (Bagci et al. 2020)
MAP3K21 (Bagci et al. 2020)
MYH11 (Bagci et al. 2020)
MYL12B (Bagci et al. 2020)
SH3RF1 (Bagci et al. 2020)
TPM3 (Bagci et al. 2020)
TPM4 (Bagci et al. 2020)
ZNF512B (Bagci et al. 2020)

Literature References
PubMed ID Title Journal Year
19064640 The transforming Rho family GTPase Wrch-1 disrupts epithelial cell tight junctions and epithelial morphogenesis

Fanning, AS, Alan, JK, Cox, AD, Madigan, JP, Brady, DC

Mol Cell Biol 2009
32737221 The atypical Rho GTPase RhoU interacts with intersectin-2 to regulate endosomal recycling pathways

Gryaznova, T, Croisé, P, Gubar, O, Gasman, S, Rynditch, A, Ory, S, Tóth, P, Blangy, A, Kropyvko, S, Vitale, N

J Cell Sci 2020
17504809 The atypical Rho family GTPase Wrch-1 regulates focal adhesion formation and cell migration

Valster, A, Chuang, YY, Coniglio, SJ, Symons, M, Backer, JM

J Cell Sci 2007
21508312 GRB2 couples RhoU to epidermal growth factor receptor signaling and cell migration

Billadeau, DD, Zhang, JS, Koenig, A, Young, C

Mol Biol Cell 2011
26598620 PAK4 promotes kinase-independent stabilization of RhoU to modulate cell adhesion

Brown, JP, Box, GM, Pinder, SE, Court, W, Wells, CM, Gale, ME, Eccles, SA, Dart, AE

J. Cell Biol. 2015
15350535 Wrch1 is a GTPase-deficient Cdc42-like protein with unusual binding characteristics and cellular effects

Wollberg, P, Saras, J, Aspenström, P

Exp. Cell Res. 2004
21565175 ARHGAP30 is a Wrch-1-interacting protein involved in actin dynamics and cell adhesion

Pacholsky, D, Naji, L, Aspenström, P

Biochem. Biophys. Res. Commun. 2011
15556869 Atypical mechanism of regulation of the Wrch-1 Rho family small GTPase

Der, CJ, Shutes, A, Cox, AD, Berzat, AC

Curr. Biol. 2004
11459829 Wrch-1, a novel member of the Rho gene family that is regulated by Wnt-1

Levine, AJ, Kalejta, RF, Pennica, D, Tao, W, Xu, L

Genes Dev. 2001
31871319 Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms

Tran, V, Gingras, AC, Elkholi, IE, Robert, A, Boulais, J, Faubert, D, Dubé, N, Hipfner, DR, Cote, JF, Lin, ZY, Bagci, H, Sriskandarajah, N, Thibault, MP

Nat. Cell Biol. 2020
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