RUNX1:CBFB, SIN3A(SIN3B), PRMT6 and HDAC1 bind the MIR27A gene promoter

Stable Identifier
Reaction [binding]
Homo sapiens
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The transcriptional co-repressor SIN3A (and possibly SIN3B) can bind to the RUNX1:CBFB complex at the promoter of the MIR27A gene, encoding microRNA miR-27a (Ben-Ami et al. 2009, Herglotz et al. 2013). Binding of SIN3A (and probably SIN3B) to RUNX1 is inhibited by PRMT1-mediated arginine methylation of RUNX1 arginine residues R206 and R210 (Zhao et al. 2008). In addition to SIN3A, the RUNX1-containing transcriptional repressor complex at the MIR27A promoter also includes histone arginine methyltransferase PRMT6 and histone deacetylase HDAC1 (Herglotz et al. 2013). Dimethylation of histone H3 on lysine residue K4 (K5 when taking into account the initiator methionine), known as the H3K4me2 mark, is characteristic of nucleosomes associated with megakaryocyte specific promoters, including the MIR27A gene, prior to the onset of differentiation (Herglotz et al. 2013).

Literature References
PubMed ID Title Journal Year
19114653 A regulatory interplay between miR-27a and Runx1 during megakaryopoiesis

Ben-Ami, O, Pencovich, N, Lotem, J, Levanon, D, Groner, Y

Proc. Natl. Acad. Sci. U.S.A. 2009
18316480 Methylation of RUNX1 by PRMT1 abrogates SIN3A binding and potentiates its transcriptional activity

Zhao, X, Jankovic, V, Gural, A, Huang, G, Pardanani, A, Menendez, S, Zhang, J, Dunne, R, Xiao, A, Erdjument-Bromage, H, Allis, CD, Tempst, P, Nimer, SD

Genes Dev. 2008
22777353 Histone arginine methylation keeps RUNX1 target genes in an intermediate state

Herglotz, J, Kuvardina, ON, Kolodziej, S, Kumar, A, Hussong, H, Grez, M, Lausen, J

Oncogene 2013
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