Reactome | Recruitment of RNA polymerase III to TFIIIB:TFIIIC:TFIIIA:Type 1 Promoter Complex

Recruitment of RNA polymerase III to TFIIIB:TFIIIC:TFIIIA:Type 1 Promoter Complex

Stable Identifier

R-HSA-76056

Type

Reaction [binding]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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Recruitment of RNA polymerase III to TFIIIB:TFIIIC:TFIIIA:Type 1 Promoter Complex

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Cross-linking experiments performed in the yeast system have shown that within the transcription initiation complex, eight RNA polymerase III subunits can be cross-linked to DNA (Bartholomew et al., 1993). The C34 subunit, which is known to be required specifically for transcription initiation but not elongation (Wang and Roeder, 1997; Werner et al., 1993), maps the furthest upstream of the transcription start site, in close proximity to Brf1-TFIIIB (Bartholomew et al., 1993). Indeed, this subunit interacts with Brf1 (Khoo et al., 1994; Werner et al., 1993). The figure illustrates this and other protein-protein contacts involving RNA polymerase III subunits and either TFIIIC or Brf1-TFIIIB subunits. The contacts identified with S. cerevisiae proteins are indicated by stippled arrows, those identified with human protein by solid arrows. Both the S. cerevisiae RNA polymerase III subunits C53 and ABC10a interact with Tfc4 ( 1999; Flores et al., 1999), and both C17 and C34 interact with Brf1. The human subunit RPC62 interacts with TIIIC63, and RPC39 with the TFIIIC subunits TFIIIC90 and TFIIIC63 (Hsieh et al., 1999a) and the Brf1-TFIIIB subunits Brf1 and TBP (Wang and Roeder, 1997). The contacts between RNA polymerase III and TFIIIC subunits are not absolutely required for transcription in vitro with the S. cerevisiae system, in which TFIIIC can be stripped from the DNA after assembly of TFIIIB without compromising transcription (Kassavetis et al., 1990) or, indeed, where transcription can be performed in the absence of TFIIIC on TATA box-containing promoters (Kassavetis et al., 1995). Nevertheless, they may contribute to the recruitment of RNA polymerase III in vivo.

Literature References

PubMed ID	Title	Journal	Year
7568218	Cloning, expression, and function of TFC5, the gene encoding the B" component of the Saccharomyces cerevisiae RNA polymerase III transcription factor TFIIIB Kassavetis, GA, Nguyen, ST, Kobayashi, R, Kumar, A, Geiduschek, EP, Pisano, M	Proc. Natl. Acad. Sci. U.S.A.	1995
9312031	Dual role of the C34 subunit of RNA polymerase III in transcription initiation Brun, I, Sentenac, A, Werner, M	EMBO J.	1997
8407894	Interaction between a complex of RNA polymerase III subunits and the 70-kDa component of transcription factor IIIB Werner, M, Chaussivert, N, Willis, IM, Sentenac, A	J. Biol. Chem.	1993
2404611	S. cerevisiae TFIIIB is the transcription initiation factor proper of RNA polymerase III, while TFIIIA and TFIIIC are assembly factors Kassavetis, GA, Braun, BR, Nguyen, LH, Geiduschek, EP	Cell	1990
9171375	Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription initiation Wang, Z, Roeder, RG	Genes Dev	1997
8423814	Orientation and topography of RNA polymerase III in transcription complexes Bartholomew, B, Durkovich, D, Kassavetis, GA, Geiduschek, EP	Mol. Cell. Biol.	1993
7995525	Conserved functional domains of the RNA polymerase III general transcription factor BRF Khoo, B, Brophy, B, Jackson, SP	Genes Dev.	1994
1946469	The small subunit of transcription factor IIF recruits RNA polymerase II into the preinitiation complex Flores, O, Lu, H, Killeen, M, Greenblatt, J, Burton, ZF, Reinberg, D	Proc. Natl. Acad. Sci. U.S.A.	1991