Retrograde traffic of mannose-6-phosphate receptors (M6PRs) from the late endosome depends on RAB9 (Lombardi et al, 1993; Riederer et al, 1994; Barbero et al, 2002; reviewed in Pfeffer, 2011). Cargo recognition at the late endosome is mediated by the RAB9-interacting protein PLIN3/TIP47, which concentrates retrograde cargo into VAMP3 RAB9 positive vesicles (Diaz et al, 1998; Carroll et al, 2001; Reddy et al, 2006; Ganley et al, 2008). RABEPK is another RAB9:GTP interacting protein that is required for retrograde transport of M6PR to the TGN (Diaz et al, 1997). At the trans-Golgi network, RAB9 and PILN3 interact with the atypical RHO GTPase related protein RHOBTB3. This interaction is required for the TGN-localization of RAB9 M6PR positive vesicles. Interaction of RAB9 with the C-terminal domain of RHOBTB3 relieves an inhibitory intramolecular interaction in RHOBTB3, allowing the N-terminal domain to achieve maximal ATP hydrolysis, which is thought to promote the release of PLIN3/TIP47 as a precursor to vesicle fusion at the TGN (Espinosa et al, 2009)
Ganley, IG, Espinosa, E, Pfeffer, SR
Díaz, E, Schimmöller, F, Pfeffer, SR
Reddy, JV, Burguete, AS, Sridevi, K, Ganley, IG, Nottingham, RM, Pfeffer, SR
Carroll, KS, Hanna, J, Simon, I, Krise, J, Barbero, P, Pfeffer, SR
Riederer, MA, Soldati, T, Shapiro, AD, Lin, J, Pfeffer, SR
Pfeffer, SR
Díaz, E, Pfeffer, SR
Lombardi, D, Soldati, T, Riederer, MA, Goda, Y, Zerial, M, Pfeffer, SR
Barbero, P, Bittova, L, Pfeffer, SR
Espinosa, EJ, Calero, M, Sridevi, K, Pfeffer, SR
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