RAB9 binds RHOBTB3, bringing late endosome-derived vesicles to the TGN

Stable Identifier
Reaction [binding]
Homo sapiens
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Retrograde traffic of mannose-6-phosphate receptors (M6PRs) from the late endosome depends on RAB9 (Lombardi et al, 1993; Riederer et al, 1994; Barbero et al, 2002; reviewed in Pfeffer, 2011). Cargo recognition at the late endosome is mediated by the RAB9-interacting protein PLIN3/TIP47, which concentrates retrograde cargo into VAMP3 RAB9 positive vesicles (Diaz et al, 1998; Carroll et al, 2001; Reddy et al, 2006; Ganley et al, 2008). RABEPK is another RAB9:GTP interacting protein that is required for retrograde transport of M6PR to the TGN (Diaz et al, 1997). At the trans-Golgi network, RAB9 and PILN3 interact with the atypical RHO GTPase related protein RHOBTB3. This interaction is required for the TGN-localization of RAB9 M6PR positive vesicles. Interaction of RAB9 with the C-terminal domain of RHOBTB3 relieves an inhibitory intramolecular interaction in RHOBTB3, allowing the N-terminal domain to achieve maximal ATP hydrolysis, which is thought to promote the release of PLIN3/TIP47 as a precursor to vesicle fusion at the TGN (Espinosa et al, 2009)
Literature References
PubMed ID Title Journal Year
9230071 A novel Rab9 effector required for endosome-to-TGN transport

Pfeffer, SR, Díaz, E, Schimmöller, F

J. Cell Biol. 1997
18195106 A syntaxin 10-SNARE complex distinguishes two distinct transport routes from endosomes to the trans-Golgi in human cells

Pfeffer, SR, Espinosa, E, Ganley, IG

J. Cell Biol. 2008
16885419 A functional role for the GCC185 golgin in mannose 6-phosphate receptor recycling

Reddy, JV, Burguete, AS, Nottingham, RM, Sridevi, K, Pfeffer, SR, Ganley, IG

Mol. Biol. Cell 2006
11359012 Role of Rab9 GTPase in facilitating receptor recruitment by TIP47

Hanna, J, Barbero, P, Carroll, KS, Pfeffer, SR, Simon, I, Krise, J

Science 2001
7909812 Lysosome biogenesis requires Rab9 function and receptor recycling from endosomes to the trans-Golgi network

Pfeffer, SR, Shapiro, AD, Riederer, MA, Soldati, T, Lin, J

J. Cell Biol. 1994
21421921 Entry at the trans-face of the Golgi

Pfeffer, SR

Cold Spring Harb Perspect Biol 2011
9590177 TIP47: a cargo selection device for mannose 6-phosphate receptor trafficking

Pfeffer, SR, Díaz, E

Cell 1998
8440258 Rab9 functions in transport between late endosomes and the trans Golgi network

Pfeffer, SR, Riederer, MA, Soldati, T, Zerial, M, Goda, Y, Lombardi, D

EMBO J. 1993
11827983 Visualization of Rab9-mediated vesicle transport from endosomes to the trans-Golgi in living cells

Barbero, P, Pfeffer, SR, Bittova, L

J. Cell Biol. 2002
19490898 RhoBTB3: a Rho GTPase-family ATPase required for endosome to Golgi transport

Espinosa, EJ, Calero, M, Sridevi, K, Pfeffer, SR

Cell 2009
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