Retrograde traffic of mannose-6-phosphate receptors (M6PRs) from the late endosome depends on RAB9 (Lombardi et al, 1993; Riederer et al, 1994; Barbero et al, 2002; reviewed in Pfeffer, 2011). Cargo recognition at the late endosome is mediated by the RAB9-interacting protein PLIN3/TIP47, which concentrates retrograde cargo into VAMP3 RAB9 positive vesicles (Diaz et al, 1998; Carroll et al, 2001; Reddy et al, 2006; Ganley et al, 2008). RABEPK is another RAB9:GTP interacting protein that is required for retrograde transport of M6PR to the TGN (Diaz et al, 1997). At the trans-Golgi network, RAB9 and PILN3 interact with the atypical RHO GTPase related protein RHOBTB3. This interaction is required for the TGN-localization of RAB9 M6PR positive vesicles. Interaction of RAB9 with the C-terminal domain of RHOBTB3 relieves an inhibitory intramolecular interaction in RHOBTB3, allowing the N-terminal domain to achieve maximal ATP hydrolysis, which is thought to promote the release of PLIN3/TIP47 as a precursor to vesicle fusion at the TGN (Espinosa et al, 2009)
Pfeffer, SR, Díaz, E, Schimmöller, F
Pfeffer, SR, Espinosa, E, Ganley, IG
Reddy, JV, Burguete, AS, Nottingham, RM, Sridevi, K, Pfeffer, SR, Ganley, IG
Hanna, J, Barbero, P, Carroll, KS, Pfeffer, SR, Simon, I, Krise, J
Pfeffer, SR, Shapiro, AD, Riederer, MA, Soldati, T, Lin, J
Pfeffer, SR
Pfeffer, SR, Díaz, E
Pfeffer, SR, Riederer, MA, Soldati, T, Zerial, M, Goda, Y, Lombardi, D
Barbero, P, Pfeffer, SR, Bittova, L
Espinosa, EJ, Calero, M, Sridevi, K, Pfeffer, SR
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