Reactome | PIAS1 SUMOylates SP3 with SUMO2

PIAS1 SUMOylates SP3 with SUMO2

Stable Identifier

R-HSA-6804468

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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PIAS1 SUMOylates SP3 with SUMO2 at lysine-551 (Sapetschnig et al. 2002, Galisson et al. 2011, Tammsalu et al. 2014, Hendriks et al. 2015). SUMOylation reduces the transcription activation capacity of the long and the short isoforms of Sp3 (Sapetschnig et al 2004). Mechanistically, SUMO attachment to Sp3 serves as a molecular beacon for the recruitment of chromatin-modifying machineries that impose epigenetic silencing (inferred from Drosophila homologs in Stielow et al. 2008a, inferred from mouse homologs in Stielow et al. 2008b).

Literature References

PubMed ID	Title	Journal	Year
21098080	A novel proteomics approach to identify SUMOylated proteins and their modification sites in human cells Galisson, F, Mahrouche, L, Courcelles, M, Bonneil, E, Meloche, S, Chelbi-Alix, MK, Thibault, P	Mol. Cell Proteomics	2011
12356736	Transcription factor Sp3 is silenced through SUMO modification by PIAS1 Sapetschnig, A, Rischitor, G, Braun, H, Doll, A, Schergaut, M, Melchior, F, Suske, G	EMBO J.	2002
24782567	Proteome-wide identification of SUMO2 modification sites Tammsalu, T, Matic, I, Jaffray, EG, Ibrahim, AF, Tatham, MH, Hay, RT	Sci Signal	2014
18617891	SUMO-modified Sp3 represses transcription by provoking local heterochromatic gene silencing Stielow, B, Sapetschnig, A, Wink, C, Krüger, I, Suske, G	EMBO Rep.	2008
18374648	Identification of SUMO-dependent chromatin-associated transcriptional repression components by a genome-wide RNAi screen Stielow, B, Sapetschnig, A, Krüger, I, Kunert, N, Brehm, A, Boutros, M, Suske, G	Mol. Cell	2008
25114211	Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli Impens, F, Radoshevich, L, Cossart, P, Ribet, D	Proc. Natl. Acad. Sci. U.S.A.	2014
26073453	System-wide identification of wild-type SUMO-2 conjugation sites Hendriks, IA, D'Souza, RC, Chang, JG, Mann, M, Vertegaal, AC	Nat Commun	2015