D-loop formation mediated by PALB2, BRCA2 and RAD51

Stable Identifier
Reaction [binding]
Homo sapiens
Strand exchange/Branch migration mediated by PALB2, BRCA2 and RAD51
Locations in the PathwayBrowser
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout
A D-loop structure is formed when complementary duplex DNA (sister chromatid) is progressively invaded by the RAD51 nucleoprotein filament, with base pairing of the invading ssDNA and the complementary sister chromatid DNA strand (Sung et al. 2003). PALB2, RAD54, RAD51 paralogs (RAD51B, RAD51C, RAD51D, XRCC2, XRCC3), and RAD51AP1 synergistically stimulate RAD51 recombinase activity, thus enhancing RAD51-mediated strand exchange and promoting the formation of D-loop structures. PALB2 simultaneously interacts with RAD51, BRCA2 and RAD51AP1 (Modesti et al. 2007, Wiese et al. 2007, Buisson et al. 2010, Dray et al. 2010). The direct BRCA1 interaction with PALB2 helps to fine-tune the localization of BRCA2 and RAD51 at DNA double-strand breaks (DSBs) (Zhang et al. 2009, Sy et al. 2009). Phosphorylation of PALB2 by ATR on serine residue S59 promotes BRCA1-PALB2 interaction and the localization of PALB2 to DNA damage sites (Buisson et al. 2017).
Literature References
PubMed ID Title Journal Year
20871616 Enhancement of RAD51 recombinase activity by the tumor suppressor PALB2

Sy, SM, Yu, X, Egelman, E, Etchin, J, Wiese, C, Tsai, MS, Liu, D, Dray, E, Chen, J, Galkin, VE, Williams, GJ, Schild, D, Hammel, M, Sung, P, Saro, D

Nat. Struct. Mol. Biol. 2010
19268590 PALB2 links BRCA1 and BRCA2 in the DNA-damage response

Ye, L, Xia, B, Cai, H, Ma, J, Wu, J, Yu, X, Zhang, F

Curr. Biol. 2009
19369211 PALB2 is an integral component of the BRCA complex required for homologous recombination repair

Sy, SM, Huen, MS, Chen, J

Proc Natl Acad Sci U S A 2009
17996711 Promotion of homologous recombination and genomic stability by RAD51AP1 via RAD51 recombinase enhancement

Shi, I, Tsai, MS, Dray, E, Groesser, T, Williams, GJ, San Filippo, J, Schild, D, Collins, DW, Sung, P, Rydberg, B, Wiese, C

Mol. Cell 2007
12912992 Rad51 recombinase and recombination mediators.

Van Komen, S, Krejci, L, Sehorn, MG, Sung, P

J Biol Chem 2003
20871615 Cooperation of breast cancer proteins PALB2 and piccolo BRCA2 in stimulating homologous recombination

Dion-Côté, AM, Xia, B, Cai, H, Launay, H, Masson, JY, Coulombe, Y, Stasiak, AZ, Stasiak, A, Buisson, R

Nat. Struct. Mol. Biol. 2010
28089683 Coupling of Homologous Recombination and the Checkpoint by ATR

Haas, W, Zou, L, Xia, B, Foo, TK, Masson, JY, Rodrigue, A, Niraj, J, Kreuzer, J, Buisson, R, Dellaire, G, Hardy, EJ, Ho, CK

Mol Cell 2017
17996710 RAD51AP1 is a structure-specific DNA binding protein that stimulates joint molecule formation during RAD51-mediated homologous recombination

Budzowska, M, Ghirlando, R, Demmers, JA, Kanaar, R, Baldeyron, C, Modesti, M

Mol. Cell 2007
Orthologous Events
Cite Us!