Association of RPA complexes with ssDNA at resected DNA DSBs

Stable Identifier
R-HSA-5693542
Type
Reaction [binding]
Species
Homo sapiens
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Human replication protein A (RPA) is a single-stranded DNA (ssDNA) binding protein complex required for DNA replication, recombination, and repair. RPA is a stable heterotrimer consisting of subunits with molecular masses of 14, 32 and 70 kDa (p14, p32 and p70, respectively). Association of RPA with ssDNA is thought to contribute to both the protection and removal of secondary structure from single-stranded DNA (ssDNA) (McIlwraith et al. 2000). The RPA complexes coat 3'-ssDNA overhangs generated by RBBP8 (CtIP)-initiated long-range resection of DNA double strand breaks (DSBs) (Sartori et al. 2007, Chen et al. 2008).

Literature References
PubMed ID Title Journal Year
11080452 Reconstitution of the strand invasion step of double-strand break repair using human Rad51 Rad52 and RPA proteins

McIlwraith, MJ, Van Dyck, E, Masson, JY, Stasiak, AZ, Stasiak, A, West, SC

J Mol Biol 2000
18171670 Cell cycle-dependent complex formation of BRCA1.CtIP.MRN is important for DNA double-strand break repair

Chen, L, Nievera, CJ, Lee, AY, Wu, X

J. Biol. Chem. 2008
17965729 Human CtIP promotes DNA end resection

Sartori, AA, Lukas, C, Coates, J, Mistrik, M, Fu, S, Bartek, J, Baer, R, Lukas, J, Jackson, SP

Nature 2007
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