Reactome | Association of RPA complexes with ssDNA at resected DNA DSBs

Association of RPA complexes with ssDNA at resected DNA DSBs

Stable Identifier

R-HSA-5693542

Type

Reaction [binding]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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Association of RPA complexes with ssDNA at resected DNA DSBs

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Human replication protein A (RPA) is a single-stranded DNA (ssDNA) binding protein complex required for DNA replication, recombination, and repair. RPA is a stable heterotrimer consisting of subunits with molecular masses of 14, 32 and 70 kDa (p14, p32 and p70, respectively). Association of RPA with ssDNA is thought to contribute to both the protection and removal of secondary structure from single-stranded DNA (ssDNA) (McIlwraith et al. 2000). The RPA complexes coat 3'-ssDNA overhangs generated by RBBP8 (CtIP)-initiated long-range resection of DNA double-strand breaks (DSBs) (Sartori et al. 2007, Chen et al. 2008).

Literature References

PubMed ID	Title	Journal	Year
18171670	Cell cycle-dependent complex formation of BRCA1.CtIP.MRN is important for DNA double-strand break repair Chen, L, Nievera, CJ, Lee, AY, Wu, X	J. Biol. Chem.	2008
11080452	Reconstitution of the strand invasion step of double-strand break repair using human Rad51 Rad52 and RPA proteins McIlwraith, MJ, Van Dyck, E, Masson, JY, Stasiak, AZ, Stasiak, A, West, SC	J Mol Biol	2000
17965729	Human CtIP promotes DNA end resection Sartori, AA, Lukas, C, Coates, J, Mistrik, M, Fu, S, Bartek, J, Baer, R, Lukas, J, Jackson, SP	Nature	2007