Reactome | POLL or POLM extends aligned DNA DSB ends to fill gaps

POLL or POLM extends aligned DNA DSB ends to fill gaps

Stable Identifier

R-HSA-5687360

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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POLL or POLM extends aligned DNA DSB ends to fill gaps

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DNA polymerases mu (POLM) and lambda (POLL) facilitate non-homologous end joining (NHEJ) of DNA double strand breaks (DSBs) by filling single strand (ss) gaps (usually 1- or 2- nucleotide gaps) present at DNA DSB ends positioned for ligation in the synaptic complex containing XRCC5:XRCC6 (Ku), PRKDC (DNA-PKcs), DCLRE1C (ARTEMIS), XRCC4:LIG4 and NHEJ1 (XLF) (Mahajan et al. 2002, Lee et al. 2004, Fan and Wu 2004, McElhinny et al. 2005, Davis et al. 2008).

Literature References

PubMed ID	Title	Journal	Year
16061182	A gradient of template dependence defines distinct biological roles for family X polymerases in nonhomologous end joining Nick McElhinny, SA, Havener, JM, Garcia-Diaz, M, Juárez, R, Bebenek, K, Kee, BL, Blanco, L, Kunkel, TA, Ramsden, DA	Mol. Cell	2005
18397950	End-bridging is required for pol mu to efficiently promote repair of noncomplementary ends by nonhomologous end joining Davis, BJ, Havener, JM, Ramsden, DA	Nucleic Acids Res.	2008
12077346	Association of DNA polymerase mu (pol mu) with Ku and ligase IV: role for pol mu in end-joining double-strand break repair Mahajan, KN, Nick McElhinny, SA, Mitchell, BS, Ramsden, DA	Mol. Cell. Biol.	2002
15451442	DNA polymerase lambda can elongate on DNA substrates mimicking non-homologous end joining and interact with XRCC4-ligase IV complex Fan, W, Wu, X	Biochem. Biophys. Res. Commun.	2004
14561766	Implication of DNA polymerase lambda in alignment-based gap filling for nonhomologous DNA end joining in human nuclear extracts Lee, JW, Blanco, L, Zhou, T, Garcia-Diaz, M, Bebenek, K, Kunkel, TA, Wang, Z, Povirk, LF	J. Biol. Chem.	2004

Participants

Input

Output

Participates

as an event of

Nonhomologous End-Joining (NHEJ) (Homo sapiens)

Catalyst Activity

DNA-directed DNA polymerase activity of p-T2609,S2612,T2638,T2647-PRKDC:XRCC5:XRCC6:p-S516,S645-DCLRE1C:XRCC4:LIG4:NHEJ1:POLL,POLM:Ligatable DNA DSB ends [nucleoplasm]

Physical Entity

p-T2609,S2612,T2638,T2647-PRKDC:XRCC5:XRCC6:p-S516,S645-DCLRE1C:XRCC4:LIG4:NHEJ1:POLL,POLM:Ligatable DNA DSB ends [nucleoplasm] (Homo sapiens)

Activity

DNA-directed DNA polymerase activity (GO:0003887)

Orthologous Events

POLL or POLM extends aligned DNA DSB ends to fill gaps (Bos taurus)

POLL or POLM extends aligned DNA DSB ends to fill gaps (Canis familiaris)

POLL or POLM extends aligned DNA DSB ends to fill gaps (Danio rerio)

POLL or POLM extends aligned DNA DSB ends to fill gaps (Dictyostelium discoideum)

POLL or POLM extends aligned DNA DSB ends to fill gaps (Gallus gallus)

POLL or POLM extends aligned DNA DSB ends to fill gaps (Mus musculus)

POLL or POLM extends aligned DNA DSB ends to fill gaps (Rattus norvegicus)