Reactome | RAD52 promotes single strand annealing at resected DNA DSBs

RAD52 promotes single strand annealing at resected DNA DSBs

Stable Identifier

R-HSA-5686642

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

Locations in the PathwayBrowser

Expand all

General

SBML | | PDF

SVG | | PPTX | SBGN

RAD52 promotes single strand annealing at resected DNA DSBs

Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

RAD52 promotes annealing of 3' ssDNA overhangs at resected DNA double strand breaks (DSBs) through complementary regions. The complementarity between the two 3' ssDNA overhangs at resected DNA DSBs exists if 3' ssDNA overhangs contain direct repeats. While single strand annealing (SSA) requires significant homology between the annealed sequences it is nonetheless mutagenic. The parts of two 3' overhanging DNA single strands at resected DSBs that lie 3' to the annealed regions become displaced as flaps and subsequently excised. This results in the deletion (loss) of the DNA sequence lying between the two regions of homology used for SSA, as well as the deletion of one of the repeats used for annealing (Parsons et al. 2000, Van Dyck et al. 2001, Singleton et al. 2002, Stark et al. 2004, Mansour et al. 2008).

Literature References

PubMed ID	Title	Journal	Year
18539610	Hierarchy of nonhomologous end-joining, single-strand annealing and gene conversion at site-directed DNA double-strand breaks Mansour, WY, Schumacher, S, Rosskopf, R, Rhein, T, Schmidt-Petersen, F, Gatzemeier, F, Haag, F, Borgmann, K, Willers, H, Dahm-Daphi, J	Nucleic Acids Res.	2008
15485900	Genetic steps of mammalian homologous repair with distinct mutagenic consequences Stark, JM, Pierce, AJ, Oh, J, Pastink, A, Jasin, M	Mol. Cell. Biol.	2004
11571269	Visualization of recombination intermediates produced by RAD52-mediated single-strand annealing Van Dyck, E, Stasiak, AZ, Stasiak, A, West, SC	EMBO Rep	2001
10921897	Precise binding of single-stranded DNA termini by human RAD52 protein. Parsons, CA, Baumann, P, Van Dyck, E, West, SC	EMBO J	2000
12370410	Structure of the single-strand annealing domain of human RAD52 protein. Singleton, MR, Wentzell, LM, Liu, Y, West, SC, Wigley, DB	Proc Natl Acad Sci U S A	2002

Participants

Input

RAD51:p-Y104-RAD52:p-RPA:ATR:ATRIP:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)

Output

RAD51:p-Y104-RAD52:p-RPA:ATR:ATRIP:DNA DSBs with annealed 3' ssDNA overhangs and displaced flaps:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)

Participant Of

hasEvent

HDR through Single Strand Annealing (SSA)

Catalyst Activity

Title

annealing activity of RAD51:p-Y104-RAD52:p-RPA:ATR:ATRIP:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm]

Physical Entity

RAD51:p-Y104-RAD52:p-RPA:ATR:ATRIP:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm]

Activity

annealing activity (0097617)

Orthologous Events

RAD52 promotes single strand annealing at resected DNA DSBs (Bos taurus)

RAD52 promotes single strand annealing at resected DNA DSBs (Caenorhabditis elegans)

RAD52 promotes single strand annealing at resected DNA DSBs (Canis familiaris)

RAD52 promotes single strand annealing at resected DNA DSBs (Danio rerio)

RAD52 promotes single strand annealing at resected DNA DSBs (Dictyostelium discoideum)

RAD52 promotes single strand annealing at resected DNA DSBs (Gallus gallus)

RAD52 promotes single strand annealing at resected DNA DSBs (Mus musculus)