RAD52 promotes single strand annealing at resected DNA DSBs

Stable Identifier
Reaction [transition]
Homo sapiens
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RAD52 promotes annealing of 3' ssDNA overhangs at resected DNA double-strand breaks (DSBs) through complementary regions. The complementarity between the two 3' ssDNA overhangs at resected DNA DSBs exists if 3' ssDNA overhangs contain direct repeats. While single strand annealing (SSA) requires significant homology between the annealed sequences it is nonetheless mutagenic. The parts of two 3' overhanging DNA single strands at resected DSBs that lie 3' to the annealed regions become displaced as flaps and subsequently excised. This results in the deletion (loss) of the DNA sequence lying between the two regions of homology used for SSA, as well as the deletion of one of the repeats used for annealing (Parsons et al. 2000, Van Dyck et al. 2001, Singleton et al. 2002, Stark et al. 2004, Mansour et al. 2008).
Literature References
PubMed ID Title Journal Year
15485900 Genetic steps of mammalian homologous repair with distinct mutagenic consequences

Pierce, AJ, Jasin, M, Oh, J, Stark, JM, Pastink, A

Mol. Cell. Biol. 2004
18539610 Hierarchy of nonhomologous end-joining, single-strand annealing and gene conversion at site-directed DNA double-strand breaks

Haag, F, Borgmann, K, Schmidt-Petersen, F, Rhein, T, Mansour, WY, Rosskopf, R, Willers, H, Schumacher, S, Gatzemeier, F, Dahm-Daphi, J

Nucleic Acids Res. 2008
11571269 Visualization of recombination intermediates produced by RAD52-mediated single-strand annealing

West, SC, Stasiak, AZ, Stasiak, A, Van Dyck, E

EMBO Rep 2001
10921897 Precise binding of single-stranded DNA termini by human RAD52 protein.

West, SC, Parsons, CA, Van Dyck, E, Baumann, P

EMBO J 2000
12370410 Structure of the single-strand annealing domain of human RAD52 protein.

West, SC, Wentzell, LM, Wigley, DB, Liu, Y, Singleton, MR

Proc Natl Acad Sci U S A 2002
Catalyst Activity

annealing activity of RAD51:p-Y104-RAD52:p-RPA:ATR:ATRIP:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm]

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