Reactome | PCMT1 transfers CH3 from AdoMet to isoAsp to form MetAsp

PCMT1 transfers CH3 from AdoMet to isoAsp to form MetAsp

Stable Identifier

R-HSA-5676966

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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PCMT1 transfers CH3 from AdoMet to isoAsp to form MetAsp

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Protein-L-isoaspartate (D-aspartate) O-methyltransferase (PCMT1, PIMT EC 2.1.1.77) transfers the methyl group from S-adenosyl-L-methionine (AdoMet) to the alpha side-chain carboxyl group of L-isoaspartyl and D-aspartatyl amino acids (Murray & Clarke 1986, Johnson et al. 1987, Galletti et al. 1988, Lowenson & Clarke 1992, The resulting methyl ester (MetAsp) undergoes spontaneous transformation to L-succinimide, which spontaneously hydrolyses to generates L-aspartyl residues or L-isoaspartyl residues. This repair process helps to maintain overall protein integrity. When PCMT1 is not present in cells, the abnormal aspartyl residues accumulate. Pcmt1 knockout mice exhibit fatal progressive epilepsy (Yamamoto et al. 1998).

Literature References

PubMed ID	Title	Journal	Year
7592526	Characterization of three cDNAs encoding two isozymes of an isoaspartyl protein carboxyl methyltransferase from human erythroid leukemia cells Takeda, R, Mizobuchi, M, Murao, K, Sato, M, Takahara, J	J. Biochem.	1995
3624258	Partial repair of deamidation-damaged calmodulin by protein carboxyl methyltransferase Johnson, BA, Langmack, EL, Aswad, DW	J. Biol. Chem.	1987