SPOP:CUL3:RBX1 ubiquitinates GLI2,3

Stable Identifier
R-HSA-5635856
Type
Reaction
Species
Homo sapiens
Compartment
nucleoplasm
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Summation

The transcriptional activity of full-length activated Ci/GLI proteins is restricted by their rapid ubiquitin-mediated degradation after initiation of Hh signaling (Ohlmeyer et al, 1998; Humke et al, 2010; Tukachinsky et al, 2010; Wen et al, 2010). Ubiquitination of Ci, GLI2 and GLI3 is mediated by the E3 ligase complex SPOP:CUL3:RBX1, which ubiquitinates the transcription factors in a Hh-dependent manner (Zhang et al, 2006; Kent et al, 2006; Zhang et al, 2009; Chen et al, 2009).

Literature References
PubMed ID Title Journal Year
16651542 Roadkill attenuates Hedgehog responses through degradation of Cubitus interruptus

Kent, D, Bush, EW, Hooper, JE

Development 2006
19955409 Multiple Ser/Thr-rich degrons mediate the degradation of Ci/Gli by the Cul3-HIB/SPOP E3 ubiquitin ligase

Zhang, Q, Shi, Q, Chen, Y, Yue, T, Li, S, Wang, B, Jiang, J

Proc. Natl. Acad. Sci. U.S.A. 2009
19684112 Cilium-independent regulation of Gli protein function by Sufu in Hedgehog signaling is evolutionarily conserved

Chen, MH, Wilson, CW, Li, YJ, Law, KK, Lu, CS, Gacayan, R, Zhang, X, Hui, CC, Chuang, PT

Genes Dev. 2009
20154143 Kinetics of hedgehog-dependent full-length Gli3 accumulation in primary cilia and subsequent degradation

Wen, X, Lai, CK, Evangelista, M, Hongo, JA, de Sauvage, FJ, Scales, SJ

Mol. Cell. Biol. 2010
16740475 A hedgehog-induced BTB protein modulates hedgehog signaling by degrading Ci/Gli transcription factor

Zhang, Q, Zhang, L, Wang, B, Ou, CY, Chien, CT, Jiang, J

Dev Cell 2006
20360384 The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteins

Humke, EW, Dorn, KV, Milenkovic, L, Scott, MP, Rohatgi, R

Genes Dev. 2010
20956384 A mechanism for vertebrate Hedgehog signaling: recruitment to cilia and dissociation of SuFu-Gli protein complexes

Tukachinsky, H, Lopez, LV, Salic, A

J. Cell Biol. 2010
9874371 Hedgehog stimulates maturation of Cubitus interruptus into a labile transcriptional activator

Ohlmeyer, JT, Kalderon, D

Nature 1998
Participants
Input
Output
Participant Of
hasEvent
Catalyst Activity
Catalyst Activity
Title
ubiquitin protein ligase activity of 2p-GLI:SPOP:CUL3:RBX1 [nucleoplasm]
Physical Entity
2p-GLI:SPOP:CUL3:RBX1 [nucleoplasm]
Activity
ubiquitin protein ligase activity (0061630)
Orthologous Events
SPOP:CUL3:RBX1 ubiquitinates GLI2,3 (Arabidopsis thaliana)
SPOP:CUL3:RBX1 ubiquitinates GLI2,3 (Bos taurus)
SPOP:CUL3:RBX1 ubiquitinates GLI2,3 (Caenorhabditis elegans)
SPOP:CUL3:RBX1 ubiquitinates GLI2,3 (Canis familiaris)
SPOP:CUL3:RBX1 ubiquitinates GLI2,3 (Danio rerio)
SPOP:CUL3:RBX1 ubiquitinates GLI2,3 (Dictyostelium discoideum)
SPOP:CUL3:RBX1 ubiquitinates GLI2,3 (Drosophila melanogaster)
SPOP:CUL3:RBX1 ubiquitinates GLI2,3 (Gallus gallus)
SPOP:CUL3:RBX1 ubiquitinates GLI2,3 (Mus musculus)
SPOP:CUL3:RBX1 ubiquitinates GLI2,3 (Oryza sativa)
SPOP:CUL3:RBX1 ubiquitinates GLI2,3 (Rattus norvegicus)
SPOP:CUL3:RBX1 ubiquitinates GLI2,3 (Schizosaccharomyces pombe)
SPOP:CUL3:RBX1 ubiquitinates GLI2,3 (Sus scrofa)
SPOP:CUL3:RBX1 ubiquitinates GLI2,3 (Taeniopygia guttata)
SPOP:CUL3:RBX1 ubiquitinates GLI2,3 (Xenopus tropicalis)
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