Reactome | TRIP11:IFT20 dissociates

TRIP11:IFT20 dissociates

Stable Identifier

R-HSA-5617828

Type

Reaction [dissociation]

Species

Homo sapiens

Compartment

Golgi membrane

ReviewStatus

5/5

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IFT20 is a member of the IFT B anterograde complex that is required for cilia formation and that, uniquely among IFT proteins, is found at the Golgi in addition to the centrosome and the cilium. Fluorescently-labelled IFT20 shuttles between the Golgi complex and the cilium and the ciliary microtubules (Follit et al, 2006; Follit et al, 2009). Golgi-association of IFT20 depends on interaction with the peripheral membrane protein TRIP11 and this interaction occurs independently of the IFT B complex (Follit et al, 2008). Golgi-localization of IFT20 is abolished in cells lacking TRIP11, and cilia in these cells are short and have a depleted complement of polycystin-2, a ciliary-localized membrane protein (Follit et al, 2008). RNAi-depletion of IFT20 in mammalian cells similarly compromises the traffic of polycystin-2 to the cilium (Follit et al, 2006). These data suggest that IFT20 may have a role at the Golgi complex in sorting and transporting membrane proteins that are destined for the cilium (Follit et al, 2006; Follit et al, 2008; Follit et al, 2009). IFT54, another IFT B component that is localized at the cilia, interacts with IFT20 but not with TRIP11, and overexpression of IFT54 displaces IFT20 from the Golgi. This supports a model where, after dissociation of the TRIP11:IFT20 complex, IFT54 docks IFT20 at the cilium, possibly on the surface of Golgi-derived vesicles, thus completing assembly of the IFT B complex and delivering ciliary membrane and membrane proteins to the site of cilium assembly (Follit et al, 2009; Omori et al, 2008; Li et al, 2008).

Literature References

PubMed ID	Title	Journal	Year
16775004	The intraflagellar transport protein IFT20 is associated with the Golgi complex and is required for cilia assembly Tuft, RA, Pazour, GJ, Follit, JA, Fogarty, KE	Mol. Biol. Cell	2006
18369462	An essential role for DYF-11/MIP-T3 in assembling functional intraflagellar transport complexes Zhen, M, Bialas, NJ, Healey, MP, Swoboda, P, Leroux, MR, Inglis, PN, Davis, EE, Héon, E, Leitch, CC, Katsanis, N, Mok, CA, Li, C, Efimenko, E, Zaghloul, NA	PLoS Genet.	2008
19253336	Characterization of mouse IFT complex B Xu, F, Pazour, GJ, Follit, JA, Keady, BT	Cell Motil. Cytoskeleton	2009
18364699	Elipsa is an early determinant of ciliogenesis that links the IFT particle to membrane-associated small GTPase Rab8 Sengupta, P, Malicki, J, Kim, W, Veraksa, A, Mukhopadhyay, S, Zhao, C, Saras, A, Omori, Y, Furukawa, T	Nat. Cell Biol.	2008
19112494	The Golgin GMAP210/TRIP11 anchors IFT20 to the Golgi complex Xu, F, Pazour, GJ, San Agustin, JT, Follit, JA, Jonassen, JA, Lo, CW, Samtani, R	PLoS Genet.	2008