Reactome | Importin-8 imports AGO2:miRNA into the nucleus

Importin-8 imports AGO2:miRNA into the nucleus

Stable Identifier

R-HSA-5578744

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol, nucleoplasm, nuclear envelope

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Importin-8 imports AGO2:miRNA into the nucleus

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The AGO2:miRNA complex is formed in the cytosol (Ohrt et al 2008) and is imported into the nucleus in a complex with Importin-8 (IPO8, Imp8, RanBP8) (Weinmann et al. 2009, Wei et al. 2014). Once in the nucleus, Imp8 in complex with the cargo interacts with RAN:GTP, causing the dissociation of Imp8 from the complex with AGO2:miRNA (Gorlich et al. 1997). Other Argonautes are also observed in the nucleus (Robb et al. 2005, Weinmann et al. 2009, Doyle et al. 2013, Ahlenstiel et al. 2012, Gagnon et al. 2014) and may be imported by the same mechanism.

Literature References

PubMed ID	Title	Journal	Year
19167051	Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs Mütze, J, Ivacevic, T, Weinmann, L, Höck, J, Urlaub, H, Meister, G, Kremmer, E, Schwille, P, Ohrt, T, Benes, V	Cell	2009
9214382	A novel class of RanGTP binding proteins Kutay, U, Bischoff, FR, Gorlich, D, Hartmann, E, Prehn, S, Bork, P, Izaurralde, E, Dabrowski, M	J Cell Biol	1997
23150874	Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus Nishi, A, Nishi, K, Nagasawa, T, Ui-Tei, K	RNA	2013
18842624	Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy reveal the cytoplasmic origination of loaded nuclear RISC in vivo in human cells Schwille, P, Mütze, J, Crell, K, Weinmann, L, Staroske, W, Höck, J, Ohrt, T, Meister, G	Nucleic Acids Res	2008
24388755	RNAi factors are present and active in human cell nuclei Chu, Y, Janowski, BA, Gagnon, KT, Li, L, Corey, DR	Cell Rep	2014
22064859	Direct evidence of nuclear Argonaute distribution during transcriptional silencing links the actin cytoskeleton to nuclear RNAi machinery in human cells Ahlenstiel, CL, Lim, HG, Suzuki, K, Kelleher, AD, Cooper, DA, Ishida, T	Nucleic Acids Res.	2012
24596094	Importin 8 regulates the transport of mature microRNAs into the cell nucleus Wei, Y, Li, L, Zen, K, Zhang, CY, Wang, D	J. Biol. Chem.	2014
15643423	Specific and potent RNAi in the nucleus of human cells Rana, TM, Khurana, J, Robb, GB, Brown, KM	Nat. Struct. Mol. Biol.	2005
23882114	The double-stranded RNA binding domain of human Dicer functions as a nuclear localization signal Filipowicz, W, Hugenschmidt, T, Badertscher, L, Jurado, S, Doyle, M, Güttinger, S, Jaskiewicz, L, Kutay, U	RNA	2013