Importin-8 imports AGO2:miRNA into the nucleus

Stable Identifier
R-HSA-5578744
Type
Reaction [transition]
Species
Homo sapiens
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The AGO2:miRNA complex is formed in the cytosol (Ohrt et al 2008) and is imported into the nucleus in a complex with Importin-8 (IPO8, Imp8, RanBP8) (Weinmann et al. 2009, Wei et al. 2014). Once in the nucleus, Imp8 in complex with the cargo interacts with RAN:GTP, causing the dissociation of Imp8 from the complex with AGO2:miRNA (Gorlich et al. 1997). Other Argonautes are also observed in the nucleus (Robb et al. 2005, Weinmann et al. 2009, Doyle et al. 2013, Ahlenstiel et al. 2012, Gagnon et al. 2014) and may be imported by the same mechanism.

Literature References
PubMed ID Title Journal Year
19167051 Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs

Mütze, J, Ivacevic, T, Weinmann, L, Höck, J, Urlaub, H, Meister, G, Kremmer, E, Schwille, P, Ohrt, T, Benes, V

Cell 2009
9214382 A novel class of RanGTP binding proteins

Kutay, U, Bischoff, FR, Gorlich, D, Hartmann, E, Prehn, S, Bork, P, Izaurralde, E, Dabrowski, M

J Cell Biol 1997
23150874 Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus

Nishi, A, Nishi, K, Nagasawa, T, Ui-Tei, K

RNA 2013
18842624 Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy reveal the cytoplasmic origination of loaded nuclear RISC in vivo in human cells

Schwille, P, Mütze, J, Crell, K, Weinmann, L, Staroske, W, Höck, J, Ohrt, T, Meister, G

Nucleic Acids Res 2008
24388755 RNAi factors are present and active in human cell nuclei

Chu, Y, Janowski, BA, Gagnon, KT, Li, L, Corey, DR

Cell Rep 2014
22064859 Direct evidence of nuclear Argonaute distribution during transcriptional silencing links the actin cytoskeleton to nuclear RNAi machinery in human cells

Ahlenstiel, CL, Lim, HG, Suzuki, K, Kelleher, AD, Cooper, DA, Ishida, T

Nucleic Acids Res. 2012
24596094 Importin 8 regulates the transport of mature microRNAs into the cell nucleus

Wei, Y, Li, L, Zen, K, Zhang, CY, Wang, D

J. Biol. Chem. 2014
15643423 Specific and potent RNAi in the nucleus of human cells

Rana, TM, Khurana, J, Robb, GB, Brown, KM

Nat. Struct. Mol. Biol. 2005
23882114 The double-stranded RNA binding domain of human Dicer functions as a nuclear localization signal

Filipowicz, W, Hugenschmidt, T, Badertscher, L, Jurado, S, Doyle, M, Güttinger, S, Jaskiewicz, L, Kutay, U

RNA 2013
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Catalyst Activity

structural constituent of nuclear pore activity of Nuclear Pore Complex (NPC) [nuclear envelope]

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