Reactome | 55S ribosome hydrolyzes peptidyl-tRNA bond and MTRF1L hydrolyzes GTP

55S ribosome hydrolyzes peptidyl-tRNA bond and MTRF1L hydrolyzes GTP

Stable Identifier

R-HSA-5419271

Type

Reaction [omitted]

Species

Homo sapiens

Compartment

mitochondrial matrix, mitochondrial inner membrane

ReviewStatus

5/5

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55S ribosome hydrolyzes peptidyl-tRNA bond and MTRF1L hydrolyzes GTP

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Binding of the MTRF1L (MTRF1a) termination factor triggers hydrolysis of the peptidyl-tRNA bond by the 39S subunit of the ribosome and release of the translated polypeptide (Soleimanpour-Lichaei et al. 2007, Nozaki et al. 2008, reviewed in Christian and Spremulli 2012). MTRF1L hydrolyzes GTP during the reaction. Stalled ribosomes are rescued by binding of an ICT1 protein in addition to the ICT1 subunit integrated in the 39S subunit (Richter et al. 2010, Akabane et al. 2014).

Literature References

PubMed ID	Title	Journal	Year
20186120	A functional peptidyl-tRNA hydrolase, ICT1, has been recruited into the human mitochondrial ribosome Lightowlers, RN, Rorbach, J, Wessels, HJ, Chrzanowska-Lightowlers, ZM, Pajak, A, Richter, R, Huynen, MA, Smeitink, JA, Smith, PM	EMBO J.	2010
25233460	Ribosome Rescue and Translation Termination at Non-Standard Stop Codons by ICT1 in Mammalian Mitochondria Nierhaus, KH, Akabane, S, Takeuchi, N, Ueda, T	PLoS Genet.	2014
18429816	HMRF1L is a human mitochondrial translation release factor involved in the decoding of the termination codons UAA and UAG Ishizawa, T, Nozaki, Y, Takeuchi, N, Matsunaga, N, Ueda, T	Genes Cells	2008
22172991	Mechanism of protein biosynthesis in mammalian mitochondria Spremulli, LL, Christian, BE	Biochim. Biophys. Acta	2012
17803939	mtRF1a is a human mitochondrial translation release factor decoding the major termination codons UAA and UAG Kühl, I, Passos, JF, Rorbach, J, Wydro, M, Chrzanowska-Lightowlers, ZM, Lightowlers, R, Temperley, R, Gaisne, M, Tate, W, Soleimanpour-Lichaei, HR, Bonnefoy, N	Mol. Cell	2007