55S ribosome hydrolyzes peptidyl-tRNA bond and MTRF1L hydrolyzes GTP

Stable Identifier
Reaction [omitted]
Homo sapiens
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Binding of the MTRF1L (MTRF1a) termination factor triggers hydrolysis of the peptidyl-tRNA bond by the 39S subunit of the ribosome and release of the translated polypeptide (Soleimanpour-Lichaei et al. 2007, Nozaki et al. 2008, reviewed in Christian and Spremulli 2012). MTRF1L hydrolyzes GTP during the reaction. Stalled ribosomes are rescued by binding of an ICT1 protein in addition to the ICT1 subunit integrated in the 39S subunit (Richter et al. 2010, Akabane et al. 2014).

Literature References
PubMed ID Title Journal Year
20186120 A functional peptidyl-tRNA hydrolase, ICT1, has been recruited into the human mitochondrial ribosome

Lightowlers, RN, Rorbach, J, Wessels, HJ, Chrzanowska-Lightowlers, ZM, Pajak, A, Richter, R, Huynen, MA, Smeitink, JA, Smith, PM

EMBO J. 2010
25233460 Ribosome Rescue and Translation Termination at Non-Standard Stop Codons by ICT1 in Mammalian Mitochondria

Nierhaus, KH, Akabane, S, Takeuchi, N, Ueda, T

PLoS Genet. 2014
18429816 HMRF1L is a human mitochondrial translation release factor involved in the decoding of the termination codons UAA and UAG

Ishizawa, T, Nozaki, Y, Takeuchi, N, Matsunaga, N, Ueda, T

Genes Cells 2008
22172991 Mechanism of protein biosynthesis in mammalian mitochondria

Spremulli, LL, Christian, BE

Biochim. Biophys. Acta 2012
17803939 mtRF1a is a human mitochondrial translation release factor decoding the major termination codons UAA and UAG

Kühl, I, Passos, JF, Rorbach, J, Wydro, M, Chrzanowska-Lightowlers, ZM, Lightowlers, R, Temperley, R, Gaisne, M, Tate, W, Soleimanpour-Lichaei, HR, Bonnefoy, N

Mol. Cell 2007
Orthologous Events
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