EXO1 exonucleolytically degrades the strand being repaired in a 5' to 3' direction (Zhang et al. 2005, Orans et al. 2011, and inferred from EXO1 activity with MSH2:MSH6) to create a single -stranded gap extending 90-170 nucleotides beyond the insertion/deletion loop (IDL) (Fang and Modrich 1993). MLH1:PMS2 limits the length of excisions by EXO1 (Zhang et al. 2005). EXO1 also forms a complex with PCNA during S phase (Liberti et al. 2011). RPA binds the resulting single-stranded DNA (Lin et al. 1998, Ramilo et al. 2002, Zhang et al. 2005, reviewed in Iftode et al. 1999).
Rasmussen, LJ, Miron, S, May, A, Bohr, VA, Liberti, SE, Wang, J, Charbonnier, JB, Andersen, SD, Keijzers, G, Nielsen, FC, Perderiset, M
Fang, WH, Modrich, P
Daniely, Y, Borowiec, JA, Iftode, C
Gao, Y, Zhang, Y, Li, GM, Yuan, F, Tian, K, Presnell, SR, Gu, L, Tomkinson, AE
Shivji, MK, Lin, YL, Wood, RD, Dutta, A, Chen, C, Kolodner, R
Patrick, SM, Guo, S, Zhang, X, Li, GM, Ramilo, C, Turchi, JJ, Gu, L
Hellinga, HW, McSweeney, EA, Modrich, P, Beese, LS, Iyer, RR, Orans, J, Hast, MA
5'-3' exonuclease activity of EXO1:MLH1:PMS2:MSH2:MSH3:ATP:PCNA:DNA containing IDL of 2 or more bases and single strand incision [nucleoplasm]
© 2022 Reactome