EXO1 exonucleolytically degrades the strand being repaired in a 5' to 3' direction (Genschel et al. 2002, Genschel and Modrich 2003, Dzantiev et al. 2004, Zhang et al. 2005, Constantin et al. 2005, Genschel and Modrich 2006, Orans et al. 2011) to create a single -stranded gap extending 90-170 nucleotides beyond the mismatch (Fang and Modrich 1993). Interaction of MSH2:MSH6 (MutSalpha) with EXO1 reduces the exonuclease activity of EXO1 on correctly paired DNA duplexes (Orans et al. 2011). MLH1:PMS2 limits the length of excisions by EXO1 (Zhang et al. 2005). EXO1 also forms a complex with PCNA during S phase (Liberti et al. 2011). RPA binds the resulting single-stranded DNA (Lin et al. 1998, Ramilo et al. 2002, Zhang et al. 2005, reviewed in Iftode et al. 1999).
Fang, WH, Modrich, P
Daniely, Y, Borowiec, JA, Iftode, C
Gao, Y, Zhang, Y, Li, GM, Yuan, F, Tian, K, Presnell, SR, Gu, L, Tomkinson, AE
Modrich, P, Genschel, J, Bazemore, LR
Shivji, MK, Lin, YL, Wood, RD, Dutta, A, Chen, C, Kolodner, R
Modrich, P, Genschel, J
Dzantiev, L, Modrich, P, Kadyrov, FA, Constantin, N
Dzantiev, L, Modrich, P, Burgers, PM, Iyer, RR, Genschel, J, Constantin, N
Patrick, SM, Guo, S, Zhang, X, Li, GM, Ramilo, C, Turchi, JJ, Gu, L
Hellinga, HW, McSweeney, EA, Modrich, P, Beese, LS, Iyer, RR, Orans, J, Hast, MA
5'-3' exonuclease activity of EXO1:MLH1:PMS2:MSH2:MSH6:ATP:PCNA:DNA containing 1-2 base mismatch and single strand incision [nucleoplasm]
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