botA HC:LC binds SV2A, B, or C and GT1b on the target cell surface

Stable Identifier
R-HSA-5244415
Type
Reaction [binding]
Species
Homo sapiens
Related Species
Clostridium botulinum
Compartment
extracellular region, plasma membrane
ReviewStatus
5/5
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botA HC:LC binds SV2A, B, or C and GT1b on the target cell surface
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The Botulinum toxin type A disulfide bonded heavy chain - light chain heterodimer (botA HC:LC, encoded by the C. botulinum botA gene) (Lacy et al. 1998) binds ganglioside GT1b and synaptic vesicle protein 2A (SV2A) on the plasma membrane of a human target cell. In vivo, this process specifically targets synapses at neuromuscular junctions, where toxin association with ganglioside may position it to bind efficiently to SV2A, SV2B, or SV2C when those proteins are exposed at the cell surface by exocytosis (Dong et al. 2006). In vitro, botA HC:LC can bind gangliosides in addition to GT1b but with lower affinity (Kozaki et al. 1998). Only GT1b binding is annotated here.
Literature References
PubMed ID Title Journal Year
16543415 SV2 is the protein receptor for botulinum neurotoxin A

Tepp, WH, Chapman, ER, Janz, R, Dean, C, Yeh, F, Dong, M, Johnson, EA

Science 2006
9712688 Ganglioside GT1b as a complementary receptor component for Clostridium botulinum neurotoxins

Mochida, S, Kamata, Y, Kozaki, S, Nishiki, T, Watarai, S

Microb. Pathog. 1998
9783750 Crystal structure of botulinum neurotoxin type A and implications for toxicity

Tepp, WH, DasGupta, BR, Lacy, DB, Cohen, AC, Stevens, RC

Nat. Struct. Biol. 1998
Participants
Input
Output
Participates
as an event of
Disease
Name Identifier Synonyms
botulism DOID:11976 Botulism (disorder), Intoxication with Clostridium botulinum toxin, Botulism, Infection due to clostridium botulinum, Botulism, Foodborne botulism, botulism, Botulism, Botulism, Botulism, Food poisoning due to Clostridium botulinum, Botulism poisoning
Authored
Gopinathrao, G  (2006-06-15)
Krupa, S  (2006-06-15)
Reviewed
Ichtchenko, K  (2007-08-03)
Thirunavukkarasu, N  (2014-11-18)
Sharma, S  (2014-11-18)
Created
D'Eustachio, P  (2014-01-18)
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