Reactome | Exosome Complex hydrolyzes mRNA by 3' to 5' exoribonuclease digestion

Exosome Complex hydrolyzes mRNA by 3' to 5' exoribonuclease digestion

Stable Identifier

R-HSA-430028

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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Exosome Complex hydrolyzes mRNA by 3' to 5' exoribonuclease digestion

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The exosome complex hydrolyzes capped, deadenylated mRNA from 3' to 5' and yields ribonucleotides having 5'-monophosphates. In yeast the Ski2-Ski3-Ski8 complex assists degradation by the exosome complex, however little is known about the function of the homologous Ski complex in mammals. Although many exosomal components contain exonuclease signatures, only two components have been shown to degrade RNA. Rrp6/PMSCL-100 has been shown to be involved in the 3’-5’ decay of nuclear mRNAs in yeast. Rrp6 may also function in the absence of the core exosomal components. The Rrp44/dDis3 component of the core exosome has been shown to possess both 3’-5’ exonuclease activity along with endonuclease activity via its PIN domain.

Literature References

PubMed ID	Title	Journal	Year
14749774	The enzymes and control of eukaryotic mRNA turnover Song, H, Parker, R	Nat Struct Mol Biol	2004
16141059	From birth to death: the complex lives of eukaryotic mRNAs Moore, MJ	Science	2005
15475613	Messenger RNA decay in mammalian cells: the exonuclease perspective Fritz, DT, Wilusz, J, Bergman, N, Wilusz, CJ, Kilpatrick, WJ	Cell Biochem Biophys	2004
19060886	Endonucleolytic RNA cleavage by a eukaryotic exosome Dziembowski, A, Séraphin, B, Tomecki, R, Lebreton, A	Nature	2008
19225159	Core exosome-independent roles for Rrp6 in cell cycle progression Graham, AC, Kiss, DL, Andrulis, ED	Mol Biol Cell	2009
19239894	The many pathways of RNA degradation Houseley, J, Tollervey, D	Cell	2009
17245413	The highways and byways of mRNA decay Garneau, NL, Wilusz, J, Wilusz, CJ	Nat Rev Mol Cell Biol	2007
15231747	A protein interaction framework for human mRNA degradation Sanderson, CM, Lehner, B	Genome Res	2004
17545563	Human cell growth requires a functional cytoplasmic exosome, which is involved in various mRNA decay pathways van Dijk, EL, Schilders, G, Pruijn, GJ	RNA	2007
11283721	The cap-to-tail guide to mRNA turnover Wormington, M, Wilusz, CJ, Peltz, SW	Nat Rev Mol Cell Biol	2001
17174896	Reconstitution, activities, and structure of the eukaryotic RNA exosome Liu, Q, Lima, CD, Greimann, JC	Cell	2006