Exosome Complex hydrolyzes mRNA by 3' to 5' exoribonuclease digestion

Stable Identifier
Reaction [transition]
Homo sapiens
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The exosome complex hydrolyzes capped, deadenylated mRNA from 3' to 5' and yields ribonucleotides having 5'-monophosphates. In yeast the Ski2-Ski3-Ski8 complex assists degradation by the exosome complex, however little is known about the function of the homologous Ski complex in mammals. Although many exosomal components contain exonuclease signatures, only two components have been shown to degrade RNA. Rrp6/PMSCL-100 has been shown to be involved in the 3’-5’ decay of nuclear mRNAs in yeast. Rrp6 may also function in the absence of the core exosomal components. The Rrp44/dDis3 component of the core exosome has been shown to possess both 3’-5’ exonuclease activity along with endonuclease activity via its PIN domain.

Literature References
PubMed ID Title Journal Year
14749774 The enzymes and control of eukaryotic mRNA turnover

Song, H, Parker, R

Nat Struct Mol Biol 2004
16141059 From birth to death: the complex lives of eukaryotic mRNAs

Moore, MJ

Science 2005
15475613 Messenger RNA decay in mammalian cells: the exonuclease perspective

Fritz, DT, Wilusz, J, Bergman, N, Wilusz, CJ, Kilpatrick, WJ

Cell Biochem Biophys 2004
19060886 Endonucleolytic RNA cleavage by a eukaryotic exosome

Dziembowski, A, Séraphin, B, Tomecki, R, Lebreton, A

Nature 2008
19225159 Core exosome-independent roles for Rrp6 in cell cycle progression

Graham, AC, Kiss, DL, Andrulis, ED

Mol Biol Cell 2009
19239894 The many pathways of RNA degradation

Houseley, J, Tollervey, D

Cell 2009
17245413 The highways and byways of mRNA decay

Garneau, NL, Wilusz, J, Wilusz, CJ

Nat Rev Mol Cell Biol 2007
15231747 A protein interaction framework for human mRNA degradation

Sanderson, CM, Lehner, B

Genome Res 2004
17545563 Human cell growth requires a functional cytoplasmic exosome, which is involved in various mRNA decay pathways

van Dijk, EL, Schilders, G, Pruijn, GJ

RNA 2007
11283721 The cap-to-tail guide to mRNA turnover

Wormington, M, Wilusz, CJ, Peltz, SW

Nat Rev Mol Cell Biol 2001
17174896 Reconstitution, activities, and structure of the eukaryotic RNA exosome

Liu, Q, Lima, CD, Greimann, JC

Cell 2006
Catalyst Activity

3'-5'-exoribonuclease activity of Exosome:SKI complex [cytosol]

Orthologous Events
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