dihydroceramide + NAD(P)H + H+ + O2 => ceramide + NAD(P)+ + H2O

Stable Identifier
Reaction [transition]
Homo sapiens
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DEGS1 (sphingolipid delta(4)-desaturase 1 / “degenerative spermatocyte homolog 1”) enzyme associated with the cytosolic face of the endoplasmic reticulum catalyzes the desaturation of dihydroceramide to form ceramide (Cadena et al. 1997; Ternes et al. 2002). The stoichiometry and cofactor requirements of the reaction are inferred from those observed in studies of ceramide synthesis in vitro catalyzed by rat liver microsomes (Michel et al. 1997). DEGS1 may also catalyze the 4-hydroxylation of dihydroceramide to form 4-hydroxysphinganine, but with low efficiency.

Literature References
PubMed ID Title Journal Year
9312549 Characterization of ceramide synthesis. A dihydroceramide desaturase introduces the 4,5-trans-double bond of sphingosine at the level of dihydroceramide

Wang, E, Merrill AH, Jr, Michel, C, van Echten-Deckert, G, Sandhoff, K, Rother, J

J Biol Chem 1997
9188692 The product of the MLD gene is a member of the membrane fatty acid desaturase family: overexpression of MLD inhibits EGF receptor biosynthesis

Kurten, RC, Cadena, DL, Gill, GN

Biochemistry 1997
11937514 Identification and characterization of a sphingolipid delta 4-desaturase family

Zahringer, U, Franke, S, Ternes, P, Heinz, E, Sperling, P

J Biol Chem 2002
Catalyst Activity

sphingolipid delta-4 desaturase activity of DEGS1 [endoplasmic reticulum membrane]

Orthologous Events
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