Clathrin internalises EPH:EFN complexes

Stable Identifier
R-HSA-3928654
Type
Reaction [omitted]
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
General
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

During endocytosis the EPH-ephrin (EFN) intact complex and, possibly, associated cytoplasmic proteins, together with the surrounding plasma membrane, can be internalized into the EPH- or EFN-expressing cell. The clathrin pathway has been linked to EFNB endocytosis. C-terminal portion of EFNB1 encodes a putative endocytosis signal, a sequence that is recognized by the clathrin-associated adapter proteins required for loading molecules into clathrin-coated pits and vesicles. Treatment of cells expressing GFP-tagged EFNB1 with soluble, recombinant EPHB1/Fc fusion protein were internalized and observed in clathrin-coated vesicles. Internalized EFNB1 co-localizes with EEA1, an endosomal marker. EFNB1 internalization is inhibited by a dominant-negative dynamin mutant and potassium depletion. These results suggest that classical, clathrin-dependent endocytosis is responsible for EFNB internalization. (Parker at al. 2004).

Literature References
PubMed ID Title Journal Year
15351694 Reverse endocytosis of transmembrane ephrin-B ligands via a clathrin-mediated pathway

Parker, M, Roberts, R, Enriquez, M, Zhao, X, Takahashi, T, Pat Cerretti, D, Daniel, T, Chen, J

Biochem. Biophys. Res. Commun. 2004
Participants
Participant Of
Orthologous Events
Authored
Reviewed
Created
Cite Us!