XIAP binds TLE

Stable Identifier
R-HSA-3322431
Type
Reaction [binding]
Species
Homo sapiens
Compartment
nucleoplasm
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XIAP binds TLE
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XIAP (X-linked inhibitor of apoptosis) has three BIR domains with known roles in the degradation of caspases and a C-terminal E3 ligase domain with both anti-apoptotic and non-apoptotic roles (Galban and Duckett, 2010; Burstein et al, 2004). The Drosophila homologue DAIP1 was recently identified in a screen in S2 cells for regulators of Wg signalling (Hanson et al, 2012). Knockdown of XIAP in HEK293 cells reduces WNT3a-induced reporter activity and expression of endogenous WNT target genes without affecting beta-catenin levels or localization. In vitro studies show that XIAP can ubiquitinate all human TLE isoforms, including the truncated isoform Amino-terminal enhancer of split (AES). TLE3 co-immunoprecipitates with XIAP from HEK293 cells in both the presence and absence of WNT signalling, consistent with a constitutive role for XIAP in TLE regulation. XIAP may act either by ubiquitinating free nuclear TLE to reduce the amount available to interact with TCF/LEFs or by ubiquitinating TLE in the context of TCF/LEF transcriptional complexes to promote its dissociation, or both. In support of the latter model, XIAP is pulled down with TCF7L2 (TCF4) in a WNT-dependent manner, and knockdown of XIAP reduces the amount of beta-catenin that co-immunoprecipitates with TCF7L2 (TCF4) upon WNT pathway activation (Hanson et al, 2012).

Literature References
PubMed ID Title Journal Year
14685266 A novel role for XIAP in copper homeostasis through regulation of MURR1

Burstein, E, Ganesh, L, Dick, RD, van De Sluis, B, Wilkinson, JC, Klomp, LW, Wijmenga, C, Brewer, GJ, Nabel, GJ, Duckett, CS

EMBO J. 2004
22304967 XIAP monoubiquitylates Groucho/TLE to promote canonical Wnt signaling

Hanson, AJ, Wallace, HA, Freeman, TJ, Beauchamp, RD, Lee, LA, Lee, E

Mol. Cell 2012
19590513 XIAP as a ubiquitin ligase in cellular signaling

Galbán, S, Duckett, CS

Cell Death Differ. 2010
Participants
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Output
Participant Of
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Orthologous Events
XIAP binds TLE (Bos taurus)
XIAP binds TLE (Canis familiaris)
XIAP binds TLE (Danio rerio)
XIAP binds TLE (Drosophila melanogaster)
XIAP binds TLE (Gallus gallus)
XIAP binds TLE (Mus musculus)
XIAP binds TLE (Rattus norvegicus)
XIAP binds TLE (Sus scrofa)
XIAP binds TLE (Xenopus tropicalis)
Authored
Rothfels, K  (2013-05-30)
Reviewed
Rajakulendran, N  (2014-01-22)
van Amerongen, R  (2014-02-15)
Kikuchi, A  (2014-04-22)
Created
Rothfels, K  (2013-05-03)