TBK1 is phosphorylated within STING:TBK1:STAT6 complex

Stable Identifier
R-HSA-3249390
Type
Reaction [transition]
Species
Homo sapiens
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TBK1 activity is regulated by phosphorylation of Ser-172 within the kinase activation loop [Kishore N et al 2002]. TBK1 phosphorylation is thought to be an autoactivation event. Biochemical analysis demonstrated that the kinase domain alone was sufficient to fully autoactivate TBK1 and was capable of phosphorylating both macromolecular and peptide substrates [Ma X et al 2012]. Furthermore, TBK1 can autophosphorylate at Ser-172 and autoactivate when overexpressed in HEK293 cells. Additionally, in co-transfection experiments wild type TBK1 associated with and phosphorylated the catalytically inactive mutant TBK1-(K38A) at Ser-172 [Clark K et al 2009]. Studies of the crystal structure of TBK1 in complex with a potent small-molecule inhibitor BX795 revealed that Ser-172 from one protomer is located in close proximity to the active site of the neighboring protomer, providing a snapshot of a potential transautoactivation reaction intermediate [Ma X et al 2012]. However, involvement of a distinct upstream activating kinase in the TBK1 phosphorylation should not be ruled out [Clark K et al 2009].

Here we show that the activation of TBK1 occurs via an autophosphorylation event, although there is no direct evidence for TBK1 phosphorylation in STAT6-mediated signaling.

Literature References
PubMed ID Title Journal Year
11839743 IKK-i and TBK-1 are enzymatically distinct from the homologous enzyme IKK-2: comparative analysis of recombinant human IKK-i, TBK-1, and IKK-2

Kishore, N, Huynh, QK, Mathialagan, S, Hall, T, Rouw, S, Creely, D, Lange, G, Caroll, J, Reitz, B, Donnelly, A, Boddupalli, H, Combs, RG, Kretzmer, K, Tripp, CS

J. Biol. Chem. 2002
22619329 Molecular basis of Tank-binding kinase 1 activation by transautophosphorylation

Ma, X, Helgason, E, Phung, QT, Quan, CL, Iyer, RS, Lee, MW, Bowman, KK, Starovasnik, MA, Dueber, EC

Proc. Natl. Acad. Sci. U.S.A. 2012
19307177 Use of the pharmacological inhibitor BX795 to study the regulation and physiological roles of TBK1 and IkappaB kinase epsilon: a distinct upstream kinase mediates Ser-172 phosphorylation and activation

Clark, K, Plater, L, Peggie, M, Cohen, P

J Biol Chem 2009
22394562 STING specifies IRF3 phosphorylation by TBK1 in the cytosolic DNA signaling pathway

Tanaka, Y, Chen, ZJ

Sci Signal 2012
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