Reactome | Transfer of SUMO1 from E1 to UBE2I (UBC9)

Transfer of SUMO1 from E1 to UBE2I (UBC9)

Stable Identifier

R-HSA-2993780

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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Transfer of SUMO1 from E1 to UBE2I (UBC9)

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SUMO1 is transferred from cysteine-173 of UBA2 to cysteine-93 of UBC9 (UBE2I) in a transthiolation reaction (Desterro et al. 1999, Okuma et al. 1999, Tatham et al. 2003, Lois and Lima 2005, Wang et al. 2007, Werner et al. 2009). The UbL domain of E1 recruits E2 into proximity for the transfer of SUMO (Lois and Lima 2005, Wang et al. 2009), RWDD3 (RSUME) increases noncovalent binding of SUMO-1 to Ubc9 and enhances Ubc9 thioester formation (Carbia-Nagashima et al. 2007).

Literature References

PubMed ID	Title	Journal	Year
19443651	Conformational transition associated with E1-E2 interaction in small ubiquitin-like modifications Wang, J, Chen, Y, Cai, S, Hu, W, Lee, B, Fukui, L	J. Biol. Chem.	2009
19107418	Performing in vitro sumoylation reactions using recombinant enzymes Moutty, MC, Melchior, F, Möller, U, Werner, A	Methods Mol. Biol.	2009
10187858	Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1 Desterro, JM, Kemp, GD, Rodriguez, MS, Hay, RT	J. Biol. Chem.	1999
9920803	In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2 Yasuda, H, Honda, R, Tsumagari, N, Ichikawa, G, Okuma, T	Biochem. Biophys. Res. Commun.	1999
17643372	The intrinsic affinity between E2 and the Cys domain of E1 in ubiquitin-like modifications Wang, J, Chen, Y, Cai, S, Hu, W, Lee, B, Song, J	Mol. Cell	2007
15660128	Structures of the SUMO E1 provide mechanistic insights into SUMO activation and E2 recruitment to E1 Lois, LM, Lima, CD	EMBO J.	2005
12641448	Role of two residues proximal to the active site of Ubc9 in substrate recognition by the Ubc9.SUMO-1 thiolester complex Tatham, MH, Chen, Y, Hay, RT	Biochemistry	2003