Transfer of SUMO1 from E1 to UBE2I (UBC9)

Stable Identifier
Reaction [transition]
Homo sapiens
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SUMO1 is transferred from cysteine-173 of UBA2 to cysteine-93 of UBC9 (UBE2I) in a transthiolation reaction (Desterro et al. 1999, Okuma et al. 1999, Tatham et al. 2003, Lois and Lima 2005, Wang et al. 2007, Werner et al. 2009). The UbL domain of E1 recruits E2 into proximity for the transfer of SUMO (Lois and Lima 2005, Wang et al. 2009),

RWDD3 (RSUME) increases noncovalent binding of SUMO-1 to Ubc9 and enhances Ubc9 thioester formation (Carbia-Nagashima et al. 2007).

Literature References
PubMed ID Title Journal Year
19443651 Conformational transition associated with E1-E2 interaction in small ubiquitin-like modifications

Wang, J, Chen, Y, Cai, S, Hu, W, Lee, B, Fukui, L

J. Biol. Chem. 2009
19107418 Performing in vitro sumoylation reactions using recombinant enzymes

Moutty, MC, Melchior, F, Möller, U, Werner, A

Methods Mol. Biol. 2009
10187858 Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1

Desterro, JM, Kemp, GD, Rodriguez, MS, Hay, RT

J. Biol. Chem. 1999
9920803 In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2

Yasuda, H, Honda, R, Tsumagari, N, Ichikawa, G, Okuma, T

Biochem. Biophys. Res. Commun. 1999
17643372 The intrinsic affinity between E2 and the Cys domain of E1 in ubiquitin-like modifications

Wang, J, Chen, Y, Cai, S, Hu, W, Lee, B, Song, J

Mol. Cell 2007
15660128 Structures of the SUMO E1 provide mechanistic insights into SUMO activation and E2 recruitment to E1

Lois, LM, Lima, CD

EMBO J. 2005
12641448 Role of two residues proximal to the active site of Ubc9 in substrate recognition by the Ubc9.SUMO-1 thiolester complex

Tatham, MH, Chen, Y, Hay, RT

Biochemistry 2003
Catalyst Activity

SUMO transferase activity of UBA2:SAE1 [nucleoplasm]

This event is regulated
Positively by