Reactome | CDK1:CCNB phosphorylates NEK9

CDK1:CCNB phosphorylates NEK9

Stable Identifier

R-HSA-2984220

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

Locations in the PathwayBrowser

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Cell Cycle (Homo sapiens)

- Cell Cycle, Mitotic (Homo sapiens)
  - M Phase (Homo sapiens)
    - Mitotic Prophase (Homo sapiens)
      - Nuclear Envelope Breakdown (Homo sapiens)
        
        Activation of NIMA Kinases NEK9, NEK6, NEK7 (Homo sapiens)
        
        CDK1:CCNB phosphorylates NEK9 (Homo sapiens)

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NEK9 functions as a homodimer and becomes catalytically active in mitosis through phosphorylation (Roig et al. 2002). While threonine T333 of NEK9 is phosphorylated in both interphase and mitotic cells (Roig et al. 2005, Bertran et al. 2011), serine residues S29, S750 and S869 of NEK9 are phosphorylated only in mitotic cells. S29, S750 and S869 sites are proline directed and match the CDK1 consensus sequence (Bertran et al. 2011). CDK1:CCNB complex was shown to phosphorylate NEK9 in vitro (Roig et al. 2002).

Literature References

PubMed ID	Title	Journal	Year
16079175	Active Nercc1 protein kinase concentrates at centrosomes early in mitosis and is necessary for proper spindle assembly Roig, J, Groen, A, Caldwell, J, Avruch, J	Mol. Biol. Cell	2005
21642957	Nek9 is a Plk1-activated kinase that controls early centrosome separation through Nek6/7 and Eg5 Bertran, MT, Sdelci, S, Regué, L, Avruch, J, Caelles, C, Roig, J	EMBO J.	2011
12101123	Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression Roig, J, Mikhailov, A, Belham, C, Avruch, J	Genes Dev.	2002