CDK1:CCNB phosphorylates NEK9

Stable Identifier
Reaction [transition]
Homo sapiens
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NEK9 functions as a homodimer and becomes catalytically active in mitosis through phosphorylation (Roig et al. 2002). While threonine T333 of NEK9 is phosphorylated in both interphase and mitotic cells (Roig et al. 2005, Bertran et al. 2011), serine residues S29, S750 and S869 of NEK9 are phosphorylated only in mitotic cells. S29, S750 and S869 sites are proline directed and match the CDK1 consensus sequence (Bertran et al. 2011). CDK1:CCNB complex was shown to phosphorylate NEK9 in vitro (Roig et al. 2002).

Literature References
PubMed ID Title Journal Year
16079175 Active Nercc1 protein kinase concentrates at centrosomes early in mitosis and is necessary for proper spindle assembly

Caldwell, J, Avruch, J, Groen, A, Roig, J

Mol. Biol. Cell 2005
21642957 Nek9 is a Plk1-activated kinase that controls early centrosome separation through Nek6/7 and Eg5

Sdelci, S, Bertran, MT, Avruch, J, Roig, J, Caelles, C, Regué, L

EMBO J. 2011
12101123 Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression

Avruch, J, Belham, C, Roig, J, Mikhailov, A

Genes Dev. 2002
Catalyst Activity

cyclin-dependent protein serine/threonine kinase activity of CCNB1,CCNB2:p-T161-CDK1 [cytosol]

Orthologous Events
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