Cytosolic NUDT5 hydrolyses ADP-ribose to R5P and AMP

Stable Identifier
Reaction [transition]
Homo sapiens
ADP-ribose + H2O => AMP + D-ribose 5-phosphate (cytosolic)
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Cytosolic NUDT5 dimer (ADP-ribose pyrophosphatase) catalyzes the hydrolysis of ADP-ribose to form AMP and D-ribose 5-phosphate. Each NUDT5 subunit is associated with three magnesium ions (Zha et al. 2006, 2008). NUDT5 also catalyzes the hydrolysis of 8-oxo-dGTP but with a strongly alkaline pH optimum (Ito et al. 2011) so the physiological relevance of this reaction is unclear and it is not annotated here.

Literature References
PubMed ID Title Journal Year
18462755 Molecular mechanism of ADP-ribose hydrolysis by human NUDT5 from structural and kinetic studies

Zha, M, Guo, Q, Zhang, Y, Yu, B, Ou, Y, Zhong, C, Ding, J

J. Mol. Biol. 2008
21389046 Cleavage of oxidized guanine nucleotide and ADP sugar by human NUDT5 protein

Ito, R, Sekiguchi, M, Setoyama, D, Nakatsu, Y, Yamagata, Y, Hayakawa, H

J. Biochem. 2011
17052728 Crystal structures of human NUDT5 reveal insights into the structural basis of the substrate specificity

Zha, M, Zhong, C, Peng, Y, Hu, H, Ding, J

J. Mol. Biol. 2006
Participant Of
Catalyst Activity
Catalyst Activity
ADP-ribose diphosphatase activity of NUDT5 dimer [cytosol]
Physical Entity
Orthologous Events
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