botE LC cleaves target cell SNAP25

Stable Identifier
Reaction [transition]
Homo sapiens
Related Species
Clostridium botulinum
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Botulinum toxin type E light chain (botE LC), in the cytosol of a target cell, catalyzes the removal of a carboxyterminal peptide from synaptosomal-associated protein 25 (SNAP25). botE LC is a zinc metalloprotease (Binz et al. 1994; Schiavo et al. 1993; Vaidyanathan et al. 1999). SNAP25 is associated with the cytosolic face of the target cell plasma membrane where it forms part of a complex required for synaptic vesicle docking and exocytosis. Its cleavage by botulinum toxin blocks synaptic vesicle fusion with the plasma membrane and neurotransmitter release and in vivo leads to a long lasting flaccid paralysis (Sudhof et al, 1993; Sudhof 2004).
Literature References
PubMed ID Title Journal Year
9886085 Proteolysis of SNAP-25 isoforms by botulinum neurotoxin types A, C, and E: domains and amino acid residues controlling the formation of enzyme-substrate complexes and cleavage

Niemann, H, Nauenburg, S, Dorries, C, Binz, T, Jahnz, M, Bade, S, Yoshino, K, Vaidyanathan, VV

J Neurochem 1999
8402889 Membrane fusion machinery: insights from synaptic proteins

Südhof, TC, Niemann, H, Jahn, R, De Camilli, P

Cell 1993
8243676 Botulinum neurotoxins serotypes A and E cleave SNAP-25 at distinct COOH-terminal peptide bonds

Mehta, PP, Schiavo, G, DasGupta, BR, Montecucco, C, Wilson, MC, Santucci, A, Jontes, J, Benfenati, F

FEBS Lett 1993
15217342 The synaptic vesicle cycle

Südhof, TC

Annu Rev Neurosci 2004
8294407 Proteolysis of SNAP-25 by types E and A botulinal neurotoxins

Südhof, TC, Link, E, Yamasaki, S, Baumeister, A, Niemann, H, Binz, T, Jahn, R, Blasi, J

J Biol Chem 1994
Catalyst Activity

metalloendopeptidase activity of botE LC:Zn2+ [cytosol]

Name Identifier Synonyms
botulism DOID:11976 Botulism (disorder), Intoxication with Clostridium botulinum toxin, Botulism, Infection due to clostridium botulinum, Botulism, Foodborne botulism, botulism, Botulism, Botulism, Botulism, Food poisoning due to Clostridium botulinum, Botulism poisoning
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