botE LC cleaves target cell SNAP25

Stable Identifier
R-HSA-194800
Type
Reaction [transition]
Species
Homo sapiens
Related Species
Clostridium botulinum
Compartment
cytosol, plasma membrane
ReviewStatus
5/5
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botE LC cleaves target cell SNAP25
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Botulinum toxin type E light chain (botE LC), in the cytosol of a target cell, catalyzes the removal of a carboxyterminal peptide from synaptosomal-associated protein 25 (SNAP25). botE LC is a zinc metalloprotease (Binz et al. 1994; Schiavo et al. 1993; Vaidyanathan et al. 1999). SNAP25 is associated with the cytosolic face of the target cell plasma membrane where it forms part of a complex required for synaptic vesicle docking and exocytosis. Its cleavage by botulinum toxin blocks synaptic vesicle fusion with the plasma membrane and neurotransmitter release and in vivo leads to a long lasting flaccid paralysis (Sudhof et al, 1993; Sudhof 2004).
Literature References
PubMed ID Title Journal Year
9886085 Proteolysis of SNAP-25 isoforms by botulinum neurotoxin types A, C, and E: domains and amino acid residues controlling the formation of enzyme-substrate complexes and cleavage

Vaidyanathan, VV, Yoshino, K, Jahnz, M, Dorries, C, Bade, S, Nauenburg, S, Niemann, H, Binz, T

J Neurochem 1999
8402889 Membrane fusion machinery: insights from synaptic proteins

Südhof, TC, De Camilli, P, Niemann, H, Jahn, R

Cell 1993
8243676 Botulinum neurotoxins serotypes A and E cleave SNAP-25 at distinct COOH-terminal peptide bonds

Schiavo, G, Santucci, A, DasGupta, BR, Mehta, PP, Jontes, J, Benfenati, F, Wilson, MC, Montecucco, C

FEBS Lett 1993
15217342 The synaptic vesicle cycle

Südhof, TC

Annu Rev Neurosci 2004
8294407 Proteolysis of SNAP-25 by types E and A botulinal neurotoxins

Binz, T, Blasi, J, Yamasaki, S, Baumeister, A, Link, E, Südhof, TC, Jahn, R, Niemann, H

J Biol Chem 1994
Participants
Input
Output
Participates
as an event of
Catalyst Activity

metalloendopeptidase activity of botE LC:Zn2+ [cytosol]

Physical Entity
botE LC:Zn2+ [cytosol] (Clostridium botulinum)
Activity
metalloendopeptidase activity (GO:0004222)
Disease
Name Identifier Synonyms
botulism DOID:11976 Botulism (disorder), Intoxication with Clostridium botulinum toxin, Botulism, Infection due to clostridium botulinum, Botulism, Foodborne botulism, botulism, Botulism, Botulism, Botulism, Food poisoning due to Clostridium botulinum, Botulism poisoning
Cross References
Mondo
Authored
Krupa, S  (2006-06-15)
Gopinathrao, G  (2006-06-15)
Reviewed
Ichtchenko, K  (2007-08-03)
Thirunavukkarasu, N  (2014-11-18)
Sharma, S  (2014-11-18)
Created
Gopinathrao, G  (2007-03-27)
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