TetraHCA is conjugated with Coenzyme A (SLC27A2 VLCS)

Stable Identifier
Reaction [transition]
Homo sapiens
TetraHCA + ATP + CoASH => 25(R) THCA-CoA + AMP + pyrophosphate + H2O (SLC27A2 "VLCS")
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TetraHCA (25(R) 3alpha,7alpha,12alpha,24(S)-tetrahydroxy-5beta-cholestanoate), coenzyme A, and ATP react to form the CoA conjugate of 25(R) TetraHCA, AMP, pyrophosphate and water. This cytosolic reaction is catalyzed by SLC27A2 (VLCS). SLC27A5 (BACS) also catalyzes this reaction; the relative contributions of the two enzymes to de novo bile acid synthesis in vivo are not certain (Mihalik et al. 2002).

Literature References
PubMed ID Title Journal Year
10479480 Human liver-specific very-long-chain acyl-coenzyme A synthetase: cDNA cloning and characterization of a second enzymatically active protein

McGuinness, MC, Watkins, PA, Wang, SJ, Steinberg, SJ

Mol Genet Metab 1999
11980911 Participation of two members of the very long-chain acyl-CoA synthetase family in bile acid synthesis and recycling

Mihalik, SJ, Smith, KD, Heinzer, AK, Watkins, PA, Wanders, RJA, Dacremont, G, Park, J, Pei, Z, Kim, DG, Steinberg, SJ, Cuebas, DA

J Biol Chem 2002
10749848 The human liver-specific homolog of very long-chain acyl-CoA synthetase is cholate:CoA ligase

Mihalik, SJ, Watkins, PA, Kim, DG, Steinberg, SJ, Cuebas, DA

J Biol Chem 2000
Catalyst Activity

long-chain fatty acid-CoA ligase activity of SLC27A2 [endoplasmic reticulum membrane]

Orthologous Events
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