RNase H-mediated degradation of the RNA strand of the -sssDNA:RNA duplex

Stable Identifier
R-HSA-182859
Type
Reaction [transition]
Species
Homo sapiens
Related Species
Human immunodeficiency virus 1
Compartment
cytosol
ReviewStatus
5/5
Locations in the PathwayBrowser
Expand all
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
RNase H-mediated degradation of the RNA strand of the -sssDNA:RNA duplex
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout
The rate of RNase H cleavage is substantially lower than the rate of DNA synthesis (Kati et al. 1992), so the product of the combined DNA synthesis and RNA degradation events catalyzed by the RT heterodimer mediating minus-strand strong stop DNA (-sssDNA) synthesis is a DNA segment still duplexed with extended viral genomic RNA fragments. In vitro, other RT heterodimers bind the remaining RNA:DNA heteroduplexes and their RNase H domains further degrade the viral genomic RNA (Wisniewski et al. 2000a, b).
Literature References
PubMed ID Title Journal Year
11035788 Unique progressive cleavage mechanism of HIV reverse transcriptase RNase H

Wisniewski, M, Balakrishnan, M, Palaniappan, C, Fay, PJ, Bambara, RA

Proc Natl Acad Sci U S A 2000
10956669 The sequential mechanism of HIV reverse transcriptase RNase H

Wisniewski, M, Balakrishnan, M, Palaniappan, C, Fay, PJ, Bambara, RA

J Biol Chem 2000
1281479 Mechanism and fidelity of HIV reverse transcriptase

Kati, WM, Johnson, KA, Jerva, LF, Anderson, KS

J Biol Chem 1992
Participants
Input
Output
Participates
as an event of
Catalyst Activity

RNA-DNA hybrid ribonuclease activity of RT [cytosol]

Physical Entity
RT [cytosol] (Human immunodeficiency virus 1)
Activity
RNA-DNA hybrid ribonuclease activity (GO:0004523)
Disease
Name Identifier Synonyms
Human immunodeficiency virus infectious disease DOID:526 HIV infection
Cross References
Mondo
Authored
Gopinathrao, G  (2006-05-19)
D'Eustachio, P  (2006-05-19)
Reviewed
Hughes, SH  (2006-10-31)
Created
Gopinathrao, G  (2006-07-19)
Cite Us!