RNase H-mediated degradation of the RNA strand of the -sssDNA:RNA duplex

Stable Identifier
R-HSA-182859
Type
Reaction [transition]
Species
Homo sapiens
Related Species
Human immunodeficiency virus 1
Compartment
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The rate of RNase H cleavage is substantially lower than the rate of DNA synthesis (Kati et al. 1992), so the product of the combined DNA synthesis and RNA degradation events catalyzed by the RT heterodimer mediating minus-strand strong stop DNA (-sssDNA) synthesis is a DNA segment still duplexed with extended viral genomic RNA fragments. In vitro, other RT heterodimers bind the remaining RNA:DNA heteroduplexes and their RNase H domains further degrade the viral genomic RNA (Wisniewski et al. 2000a, b).

Literature References
PubMed ID Title Journal Year
10956669 The sequential mechanism of HIV reverse transcriptase RNase H

Wisniewski, M, Balakrishnan, M, Palaniappan, C, Fay, PJ, Bambara, RA

J Biol Chem 2000
11035788 Unique progressive cleavage mechanism of HIV reverse transcriptase RNase H

Wisniewski, M, Balakrishnan, M, Palaniappan, C, Fay, PJ, Bambara, RA

Proc Natl Acad Sci U S A 2000
1281479 Mechanism and fidelity of HIV reverse transcriptase

Kati, WM, Johnson, KA, Jerva, LF, Anderson, KS

J Biol Chem 1992
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
RNA-DNA hybrid ribonuclease activity of RT [cytosol]
Physical Entity
Activity
Disease
Name Identifier Synonyms
Human immunodeficiency virus infectious disease 526 HIV infection
Authored
Reviewed
Created
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