RNase H-mediated degradation of the template strand

Stable Identifier
R-HSA-182795
Type
Reaction [transition]
Species
Homo sapiens
Related Species
Human immunodeficiency virus 1
Compartment
Locations in the PathwayBrowser
General
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The rate of RNase H cleavage is substantially lower than the rate of DNA synthesis (Kati et al. 1992), so the product of the combined DNA synthesis and RNA degradation events catalyzed by the RT heterodimer mediating minus-strand DNA synthesis is a DNA segment still duplexed with extended viral genomic RNA fragments. Other RT heterodimers bind the remaining RNA:DNA heteroduplexes and their RNase H domains further degrade the viral genomic RNA (Wisniewski et al. 2000a, b). Two PPT (polypurine tract) sequence motifs in the template, one immediately 5' to the U3 sequence and one located within the pol gene in the center of the viral genome, are spared from degradation (Charneau et al. 1992; Julias et al. 2004; Pullen et al. 1993).

Literature References
PubMed ID Title Journal Year
11035788 Unique progressive cleavage mechanism of HIV reverse transcriptase RNase H

Wisniewski, M, Balakrishnan, M, Palaniappan, C, Fay, PJ, Bambara, RA

Proc Natl Acad Sci U S A 2000
15542682 Effects of mutations in the G tract of the human immunodeficiency virus type 1 polypurine tract on virus replication and RNase H cleavage

Julias, JG, McWilliams, MJ, Sarafianos, SG, Alvord, WG, Arnold, E, Hughes, SH

J Virol 2004
7681062 The sequence features important for plus strand priming by human immunodeficiency virus type 1 reverse transcriptase

Pullen, KA, Rattray, AJ, Champoux, JJ

J Biol Chem 1993
10956669 The sequential mechanism of HIV reverse transcriptase RNase H

Wisniewski, M, Balakrishnan, M, Palaniappan, C, Fay, PJ, Bambara, RA

J Biol Chem 2000
1560526 A second origin of DNA plus-strand synthesis is required for optimal Human Immunodeficiency Virus replication

Alizon, M, Charneau, P, Clavel, F

J Virol 1992
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
RNA-DNA hybrid ribonuclease activity of RTC with extensive RNase-H digestion [cytosol]
Physical Entity
Activity
Disease
Name Identifier Synonyms
Human immunodeficiency virus infectious disease 526 HIV infection
Authored
Reviewed
Created
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