The first step in the removal of the flap intermediate is the binding of Replication Protein A (RPA) to the long flap structure. RPA is a eukaryotic single-stranded DNA binding protein (Bae et al. 2001). Binding of RPA to the single strand DNA during telomeric strand displacement synthesis is necessary for the recruitment of DNA2. DNA2 is a helicase/endonuclease that resolves G quadruplexes (G4), which are DNA structures that commonly form in polyguanine-rich telomeric DNA sequences (Masuda-Sasa et al. 2008). DNA2 also removes the initiator RNA primers of Okazaki fragments (Bae et al. 2001).