Removal of RNA primer and dissociation of RPA and Dna2 from the C-strand

Stable Identifier
R-HSA-174441
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nucleoplasm
ReviewStatus
5/5
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Removal of RNA primer and dissociation of RPA and Dna2 from the C-strand
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The DNA2 endonuclease removes the initiator RNA along with several downstream deoxyribonucleotides. The cleavage of the single-stranded RNA substrate results in the disassembly of RPA and DNA2. The current data for the role of the DNA2 endonuclease has been derived from studies of yeast (Budd et al. 2000, Bae et al. 2001) and Xenopus DNA2 (Liu et al. 2000, Liao et al. 2008). DNA2-mediated cleavage of G-quadruplexes (G4), DNA structures commonly formed by polyguanine-rich telomeric DNA sequences, is necessary for completion of telomeric DNA synthesis (Masuda-Sasa et al. 2008, Lin et al. 2013).
Literature References
PubMed ID Title Journal Year
23604072 Mammalian DNA2 helicase/nuclease cleaves G-quadruplex DNA and is required for telomere integrity

Campbell, J, Dai, H, Hu, J, Sampathi, S, Shin-Ya, K, Zheng, L, Zhou, M, Huang, Q, Liu, C, Lin, W, Chai, W, Shen, B

EMBO J. 2013
18593712 Processing of G4 DNA by Dna2 helicase/nuclease and replication protein A (RPA) provides insights into the mechanism of Dna2/RPA substrate recognition

Peng, XP, Masuda-Sasa, T, Polaczek, P, Chen, L, Campbell, JL

J. Biol. Chem. 2008
Participants
Input
Output
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as an event of
Event Information
Go Biological Process
telomere maintenance via semi-conservative replication (0032201)
Catalyst Activity

5'-flap endonuclease activity of Processive complex loaded on telomere:Okazaki fragment:Flap:RPA heterotrimer:DNA2 [nucleoplasm]

Physical Entity
Processive complex loaded on telomere:Okazaki fragment:Flap:RPA heterotrimer:DNA2 [nucleoplasm] (Homo sapiens)
Activity
5'-flap endonuclease activity (GO:0017108)
Orthologous Events
Removal of RNA primer and dissociation of RPA and Dna2 from the C-strand (Bos taurus)
Removal of RNA primer and dissociation of RPA and Dna2 from the C-strand (Canis familiaris)
Removal of RNA primer and dissociation of RPA and Dna2 from the C-strand (Mus musculus)
Removal of RNA primer and dissociation of RPA and Dna2 from the C-strand (Rattus norvegicus)
Removal of RNA primer and dissociation of RPA and Dna2 from the C-strand (Sus scrofa)
Authored
Blackburn, EH  (2006-03-10)
Seidel, J  (2006-03-10)
Reviewed
Hayashi, MT  (2020-04-29)
Price, C  (2006-07-13)
Created
Gillespie, ME  (2006-02-18)
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