The primase component of DNA polymerase:primase synthesizes a 6-10 nucleotide RNA primer on the G strand of the telomere

Stable Identifier
R-HSA-174425
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nucleoplasm
ReviewStatus
5/5
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The primase component of DNA polymerase:primase synthesizes a 6-10 nucleotide RNA primer on the G strand of the telomere
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The complementary C-strand at telomeres is synthesized by the DNA polymerase alpha:primase complex (Nakamura et al. 2005) using conventional RNA priming (Wang et al. 1984). Interaction of the DNA polymerase alpha complex with the G-strand-bound CST complex is needed for successful priming of the C-strand (Feng et al. 2018).
Literature References
PubMed ID Title Journal Year
16314528 Alterations of DNA and chromatin structures at telomeres and genetic instability in mouse cells defective in DNA polymerase alpha

Nakamura, M, Nabetani, A, Mizuno, T, Hanaoka, F, Ishikawa, F

Mol. Cell. Biol. 2005
30026550 CTC1-STN1 terminates telomerase while STN1-TEN1 enables C-strand synthesis during telomere replication in colon cancer cells

Feng, X, Hsu, SJ, Bhattacharjee, A, Wang, Y, Diao, J, Price, CM

Nat Commun 2018
6693436 DNA primase from KB cells. Characterization of a primase activity tightly associated with immunoaffinity-purified DNA polymerase-alpha.

Wang, TS, Hu, SZ, Korn, D

J Biol Chem 1984
Participants
Input
Output
Participates
as an event of
Event Information
Go Biological Process
telomere maintenance via semi-conservative replication (0032201)
Catalyst Activity

DNA-directed RNA polymerase activity of POLA:primase:G-strand extended telomere end [nucleoplasm]

Physical Entity
POLA:primase:G-strand extended telomere end [nucleoplasm] (Homo sapiens)
Activity
DNA-directed RNA polymerase activity (GO:0003899)
This event is regulated
Positively by
Regulator
CST complex [nucleoplasm] (Homo sapiens)
Orthologous Events
The primase component of DNA polymerase:primase synthesizes a 6-10 nucleotide RNA primer on the G strand of the telomere (Bos taurus)
The primase component of DNA polymerase:primase synthesizes a 6-10 nucleotide RNA primer on the G strand of the telomere (Canis familiaris)
The primase component of DNA polymerase:primase synthesizes a 6-10 nucleotide RNA primer on the G strand of the telomere (Danio rerio)
The primase component of DNA polymerase:primase synthesizes a 6-10 nucleotide RNA primer on the G strand of the telomere (Gallus gallus)
The primase component of DNA polymerase:primase synthesizes a 6-10 nucleotide RNA primer on the G strand of the telomere (Mus musculus)
The primase component of DNA polymerase:primase synthesizes a 6-10 nucleotide RNA primer on the G strand of the telomere (Rattus norvegicus)
The primase component of DNA polymerase:primase synthesizes a 6-10 nucleotide RNA primer on the G strand of the telomere (Sus scrofa)
Authored
Blackburn, EH  (2006-03-10)
Seidel, J  (2006-03-10)
Reviewed
Price, C  (2006-07-13)
Hayashi, MT  (2020-04-29)
Created
Gillespie, ME  (2006-02-17)
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