Reactome | PI3P is phosphorylated to PI(3,5)P2 by PIKFYVE at the Golgi membrane

PI3P is phosphorylated to PI(3,5)P2 by PIKFYVE at the Golgi membrane

Stable Identifier

R-HSA-1675921

Type

Reaction [transition]

Species

Homo sapiens

Compartment

Golgi membrane, cytosol

ReviewStatus

5/5

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PI3P is phosphorylated to PI(3,5)P2 by PIKFYVE at the Golgi membrane

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At the Golgi membrane, the PAS complex, consisting of FYVE finger-containing phosphoinositide kinase (PIKFYVE), yeast VAC14 homologue (VAC14), and polyphosphoinositide phosphatase aka SAC3 (FIG4), binds to the membrane via PIKFYVE's FYVE finger (Sbrissa et al. 2002). The PIKFYVE kinase component phosphorylates phosphatidylinositol 3-phosphate (PI3P) to phosphatidylinositol 3,5-bisphosphate PI(3,5)P2 (Sbrissa et al. 1999, McEwen et al. 1999). The PAS complex is present in the cytosol and is recruited to the membrane (Sbrissa et al. 2007). VAC14 acts as a scaffolding protein via its C-terminal domain (Sbrissa et al. 2008).

Literature References

PubMed ID	Title	Journal	Year
11706043	Phosphatidylinositol 3-phosphate-interacting domains in PIKfyve. Binding specificity and role in PIKfyve. Endomenbrane localization Sbrissa, D, Shisheva, A, Ikonomov, OC	J Biol Chem	2002
10567352	Complementation analysis in PtdInsP kinase-deficient yeast mutants demonstrates that Schizosaccharomyces pombe and murine Fab1p homologues are phosphatidylinositol 3-phosphate 5-kinases McEwen, RK, Painter, GF, Ohya, Y, Michell, RH, Shisheva, A, Holmes, AB, Parker, PJ, Dove, SK, Cooke, FT	J Biol Chem	1999
10419465	PIKfyve, a mammalian ortholog of yeast Fab1p lipid kinase, synthesizes 5-phosphoinositides. Effect of insulin Sbrissa, D, Shisheva, A, Ikonomov, OC	J Biol Chem	1999
17556371	Core protein machinery for mammalian phosphatidylinositol 3,5-bisphosphate synthesis and turnover that regulates the progression of endosomal transport. Novel Sac phosphatase joins the ArPIKfyve-PIKfyve complex Sbrissa, D, Ijuin, T, Gruenberg, J, Fu, Z, Shisheva, A, Takenawa, T, Ikonomov, OC	J Biol Chem	2007
18950639	ArPIKfyve homomeric and heteromeric interactions scaffold PIKfyve and Sac3 in a complex to promote PIKfyve activity and functionality Sbrissa, D, Shisheva, A, Ikonomov, OC, Fenner, H	J Mol Biol	2008

Participants

Input

Output

Participates

as an event of

Synthesis of PIPs at the Golgi membrane (Homo sapiens)

Catalyst Activity

1-phosphatidylinositol-3-phosphate 5-kinase activity of PIKFYVE:VAC14:FIG4 [Golgi membrane]

Physical Entity

PIKFYVE:VAC14:FIG4 [Golgi membrane] (Homo sapiens)

Activity

1-phosphatidylinositol-3-phosphate 5-kinase activity (GO:0000285)

Inferred From

PI3P is phosphorylated to PI(3,5)P2 by Pikfyve at the Golgi membrane (Mus musculus)

Cross References

Rhea

13609

Authored

Williams, MG (2011-10-18)

Reviewed

Wakelam, M (2012-05-14)

Created

Williams, MG (2011-10-17)