RNase H-mediated cleavage of the RNA strand of the -sssDNA:RNA duplex

Stable Identifier
R-HSA-164519
Type
Reaction [transition]
Species
Homo sapiens
Related Species
Human immunodeficiency virus 1
Compartment
Locations in the PathwayBrowser
General
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

As the reverse transcriptase activity of the HIV-1 RT heterodimer catalyzes the synthesis of minus-strand strong stop DNA (-sssDNA), the RNaseH activity of the same RT heterodimer catalyzes the degradation of the complementary viral genomic RNA sequences. Degradation of this RNA is required for the efficient transfer of the -sssDNA to the 5' end of the viral genomic RNA. The RNase H active site is positioned within the HIV-1 RT heterodimer so as to attack the RNA strand of the RNA:DNA duplex at a point 18 bases behind the site of reverse transcription (Furfine and Reardon 1991; Ghosh et al. 1995; Gopalakrishnan et al. 1992; Wohrl and Moelling 1990). The rate of RNase H cleavage is substantially lower than the rate of DNA synthesis, however (Kati et al. 1992), and may further depend on RT stalling and structural features of the viral genomic RNA template. The product of these combined DNA synthesis and RNA degradation events is a DNA strand still duplexed with extended viral genomic RNA fragments.

Literature References
PubMed ID Title Journal Year
10723025 The HIV-1 reverse transcription (RT) process as target for RT inhibitors

Jonckheere, H, Anne, J, De Clercq, E

Med Res Rev 2000
7535765 Truncating alpha-helix E' of p66 human immunodeficiency virus reverse transcriptase modulates RNase H function and impairs DNA strand transfer

Ghosh, M, Howard, KJ, Cameron, CE, Benkovic, SJ, Hughes, SH, Le Grice, SF

J Biol Chem 1995
1702425 Reverse transcriptase.RNase H from the human immunodeficiency virus. Relationship of the DNA polymerase and RNA hydrolysis activities.

Furfine, ES, Reardon, JE

J Biol Chem 1991
  Retroviruses

Coffin, JM, Hughes, SH, Varmus, HE

  1997
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
RNA-DNA hybrid ribonuclease activity of RTC with minus sssDNA:tRNA primer:RNA template [cytosol]
Physical Entity
Activity
Disease
Name Identifier Synonyms
Human immunodeficiency virus infectious disease 526 HIV infection
Authored
Reviewed
Created
Cite Us!