Oxidative dealkylation of 1-EtA damaged DNA by ABH3

Stable Identifier
R-HSA-112125
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nucleoplasm
ReviewStatus
5/5
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Oxidative dealkylation of 1-EtA damaged DNA by ABH3
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ALKBH3, a homolog of E.coli AlkB (Trewick et al. 2002), removes the ethyl group from 1-ethyladenine (1-etA) in a reaction dependent on alpha-ketoglutarate, oxygen and Fe2+. ALKBH3 directly reverses alkylating damage of DNA in the form of 1-etA, releasing acetaldehyde (Duncan et al. 2002). The reversal of alkylating damage of dsDNA by ALKBH3 requires the presence of DNA helicase ASCC3, a component of the activating signal co-activator complex (Dango et al. 2011). ALKBH3 can also repair methylated RNA (Aas et al. 2003).
Literature References
PubMed ID Title Journal Year
12486230 Reversal of DNA alkylation damage by two human dioxygenases.

Duncan, T, Trewick, SC, Koivisto, P, Bates, PA, Lindahl, T, Sedgwick, B

Proc Natl Acad Sci U S A 2002
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Catalyst Activity

dioxygenase activity of ALKBH3:Fe2+:ASCC1:ASCC2:ASCC3:1-etA-dsDNA [nucleoplasm]

Physical Entity
ALKBH3:Fe2+:ASCC1:ASCC2:ASCC3:1-etA-dsDNA [nucleoplasm] (Homo sapiens)
Activity
dioxygenase activity (GO:0051213)
Authored
Pegg, AE  (2004-02-04)
Reviewed
Joshi-Tope, G  (2004-02-04)
Gillespie, ME  (2015-02-06)
Created
Joshi-Tope, G  (2004-04-09)
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