POLB incorporates the first 3' dNMP and displaces 5'ddRP at SSB site

Stable Identifier
R-HSA-111253
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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DNA polymerase beta (POLB) cannot excise oxidatively damaged 5' AP (apurinic/apyrimidinic) dideoxyribose residue (5'ddRP) at the APEX1-generated single strand break (SSB). Instead, POLB incorporates the first nucleotide (dNMP) at the 3' end of the SSB, which displaces 5'ddRP (Podlutsky et al. 2001).

Literature References
PubMed ID Title Journal Year
11250913 Human DNA polymerase beta initiates DNA synthesis during long-patch repair of reduced AP sites in DNA.

Podlutsky, AJ, Dianova, II, Podust, VN, Bohr, VA, Dianov, GL

EMBO J 2001
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
DNA-directed DNA polymerase activity of POLB:APEX1:SSB(5'ddRP)-dsDNA [nucleoplasm]
Physical Entity
Activity
Orthologous Events
Authored
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Created