POLD,POLE-mediated DNA strand displacement synthesis

Stable Identifier
R-HSA-110368
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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Polymerase delta complex (POLD) or polymerase epsilon complex (POLE) bound to PCNA-associated replication complex that also includes RPA and RFC complexes, catalyzes DNA strand displacement synthesis in a reaction facilitated by FEN1 and APEX1. POLD or POLE extend the 3' end of the single strand break (SSB) by adding up to 10 nucleotides. The 5' end of the SSB, which contains the oxidatively damaged AP (apurinic/apyrimidinic) dideoxyribose phosphate residue (5'ddRP) that could not be excised by POLB, is displaced as a flap structure by the joint action of FEN1 and POLD or POLE (Klungland and Lindahl 1997, Stucki et al. 1998, Dianov et al. 1999, Podlutsky et al. 2001, Ranalli et al. 2002).

Literature References
PubMed ID Title Journal Year
9780000 Mammalian base excision repair by DNA polymerases delta and epsilon

Stucki, M, Pascucci, B, Parlanti, E, Fortini, P, Wilson, SH, Hübscher, U, Dogliotti, E

Oncogene 1998
10460157 Replication protein A stimulates proliferating cell nuclear antigen-dependent repair of abasic sites in DNA by human cell extracts.

Dianov, GL, Jensen, BR, Kenny, MK, Bohr, VA

Biochemistry 1999
11250913 Human DNA polymerase beta initiates DNA synthesis during long-patch repair of reduced AP sites in DNA.

Podlutsky, AJ, Dianova, II, Podust, VN, Bohr, VA, Dianov, GL

EMBO J 2001
12200445 AP endonuclease 1 coordinates flap endonuclease 1 and DNA ligase I activity in long patch base excision repair.

Ranalli, TA, Tom, S, Bambara, RA

J Biol Chem 2002
9214649 Second pathway for completion of human DNA base excision-repair: reconstitution with purified proteins and requirement for DNase IV (FEN1).

Klungland, A, Lindahl, T

EMBO J 1997
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
DNA-directed DNA polymerase activity of APEX1:FEN1:PCNA:POLD,POLE:RPA:RFC:SSB(3'dNMP-displaced 5'ddRP)-dsDNA [nucleoplasm]
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Activity
Orthologous Events
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