Insertion of correct bases opposite the lesion by POLH

Stable Identifier
R-HSA-110317
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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DNA polymerase eta (POLH) correctly incorporates two adenine deoxyribonucleotides (dAMPs) opposite a TT-CPD (thymine-thymine cyclobutane pyrimidine dimer) lesion. POLH can bypass other types of lesions, such as AP sites and cisplatin-induced intrastrand cross-linked gunanines, preferentially incorporating dAMPs and dGMPs opposite the lesion. While POLH is accurate in translesion synthesis (TLS) across thymine dimers, POLH has a low fidelity in TLS across other DNA damage types and when copying undamaged DNA. One of the protective mechanisms against POLH-induced mutagenesis may be that POLH cannot continue chain elongation after an incorrect nucleotide is incorporated (Matsuda et al. 2000, Masutani et al. 2000).

Literature References
PubMed ID Title Journal Year
10801132 Low fidelity DNA synthesis by human DNA polymerase-eta

Matsuda, T, Bebenek, K, Masutani, C, Hanaoka, F, Kunkel, TA

Nature 2000
10856253 Mechanisms of accurate translesion synthesis by human DNA polymerase eta.

Masutani, C, Kusumoto, R, Iwai, S, Hanaoka, F

EMBO J 2000
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
DNA-directed DNA polymerase activity of POLH:MonoUb:K164-PCNA:RPA:RFC:TT-CPD-DNA Template [nucleoplasm]
Physical Entity
Activity
Orthologous Events
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Reviewed
Created