Cleavage of cytosine glycol by NTHL1 glycosylase

Stable Identifier
R-HSA-110226
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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NTHL1 (hNTH1; endonuclease III-like protein 1) acts as a DNA glycosylase to cleave cytosine glycol (5,6-dihydroxycytosine), a product of cytosine oxidation, from the DNA sugar-phosphate backbone, creating an apurinic/apyrimidinic (AP) site (Dizdaroglu et al. 1999). After the AP site is created, NTHL1 can act as a beta lyase to cleave the DNA strand 5' to the AP site (Ikeda et al. 1998).

Literature References
PubMed ID Title Journal Year
9890904 Excision of products of oxidative DNA base damage by human NTH1 protein

Dizdaroglu, M, Karahalil, B, Sentürker, S, Buckley, TJ, Roldán-Arjona, T

Biochemistry 1999
9705289 Purification and characterization of human NTH1, a homolog of Escherichia coli endonuclease III. Direct identification of Lys-212 as the active nucleophilic residue

Ikeda, S, Biswas, T, Roy, R, Izumi, T, Boldogh, I, Kurosky, A, Sarker, AH, Seki, S, Mitra, S

J. Biol. Chem. 1998
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity of NTHL1:Cg-dsDNA [nucleoplasm]
Physical Entity
Activity
Orthologous Events
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Created