Cleavage of cytosine glycol by NTHL1 glycosylase

Stable Identifier
Reaction [transition]
Homo sapiens
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NTHL1 (hNTH1; endonuclease III-like protein 1) acts as a DNA glycosylase to cleave cytosine glycol (5,6-dihydroxycytosine), a product of cytosine oxidation, from the DNA sugar-phosphate backbone, creating an apurinic/apyrimidinic (AP) site (Dizdaroglu et al. 1999). After the AP site is created, NTHL1 can act as a beta lyase to cleave the DNA strand 5' to the AP site (Ikeda et al. 1998).

Literature References
PubMed ID Title Journal Year
9705289 Purification and characterization of human NTH1, a homolog of Escherichia coli endonuclease III. Direct identification of Lys-212 as the active nucleophilic residue

Seki, S, Roy, R, Izumi, T, Ikeda, S, Kurosky, A, Sarker, AH, Biswas, T, Boldogh, I, Mitra, S

J. Biol. Chem. 1998
9890904 Excision of products of oxidative DNA base damage by human NTH1 protein

Sentürker, S, Dizdaroglu, M, Buckley, TJ, Roldán-Arjona, T, Karahalil, B

Biochemistry 1999
Catalyst Activity

oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity of NTHL1:Cg-dsDNA [nucleoplasm]

Orthologous Events
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