Reactome | Cleavage of cytosine glycol by NTHL1 glycosylase

Cleavage of cytosine glycol by NTHL1 glycosylase

Stable Identifier

R-HSA-110226

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

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Cleavage of cytosine glycol by NTHL1 glycosylase

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NTHL1 (hNTH1; endonuclease III-like protein 1) acts as a DNA glycosylase to cleave cytosine glycol (5,6-dihydroxycytosine), a product of cytosine oxidation, from the DNA sugar-phosphate backbone, creating an apurinic/apyrimidinic (AP) site (Dizdaroglu et al. 1999). After the AP site is created, NTHL1 can act as a beta lyase to cleave the DNA strand 5' to the AP site (Ikeda et al. 1998).

Literature References

PubMed ID	Title	Journal	Year
9890904	Excision of products of oxidative DNA base damage by human NTH1 protein Dizdaroglu, M, Karahalil, B, Sentürker, S, Buckley, TJ, Roldán-Arjona, T	Biochemistry	1999
9705289	Purification and characterization of human NTH1, a homolog of Escherichia coli endonuclease III. Direct identification of Lys-212 as the active nucleophilic residue Ikeda, S, Biswas, T, Roy, R, Izumi, T, Boldogh, I, Kurosky, A, Sarker, AH, Seki, S, Mitra, S	J. Biol. Chem.	1998