5'-phosphoribosyl-5-aminoimidazole-4-carboxylate (CAIR) + L-aspartate + ATP => 5'-phosphoribosyl-5-aminoimidazole-4-N-succinocarboxamide (SAICAR) + adenosine 5'-diphosphate + orthophosphate

Stable Identifier
R-GGA-419223
Type
Reaction [transition]
Species
Gallus gallus
Compartment
cytosol
ReviewStatus
5/5
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5'-phosphoribosyl-5-aminoimidazole-4-carboxylate (CAIR) + L-aspartate + ATP => 5'-phosphoribosyl-5-aminoimidazole-4-N-succinocarboxamide (SAICAR) + adenosine 5'-diphosphate + orthophosphate
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The phosphoribosylaminoimidazolesuccinocarboxamide synthase activity of cytosolic PAICS (multifunctional protein ADE2) catalyzes the reaction of 5'-phosphoribosyl-5-aminoimidazole-4-carboxylate (CAIR), L-aspartate, and ATP to form 5'-phosphoribosyl-5-aminoimidazole-4-N-succinocarboxamide (SAICAR), ADP, and orthophosphate. Chicken PAICS protein has been purified and its catalytic properties analyzed in vitro (Firestine and Davisson 1994). The native chicken protein behaves as a large multimer on gel filtration; it is inferred to be a octamer by analogy to the human protein (Li et al. 2007). The subcellular location of the chicken enzyme is inferred from that of its better-characterized mammalian counterparts.
Literature References
PubMed ID Title Journal Year
7918410 Carboxylases in de novo purine biosynthesis. Characterization of the Gallus gallus bifunctional enzyme

Firestine, SM, Davisson, VJ

Biochemistry 1994
17224163 Octameric structure of the human bifunctional enzyme PAICS in purine biosynthesis

Li, SX, Tong, YP, Xie, XC, Wang, QH, Zhou, HN, Han, Y, Zhang, ZY, Gao, W, Li, SG, Zhang, XC, Bi, RC

J Mol Biol 2007
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Catalyst Activity

phosphoribosylaminoimidazolesuccinocarboxamide synthase activity of PAICS octamer [cytosol]

Physical Entity
PAICS octamer [cytosol] (Gallus gallus)
Activity
phosphoribosylaminoimidazolesuccinocarboxamide synthase activity (GO:0004639)
Authored
D'Eustachio, P  (2009-04-29)
Created
D'Eustachio, P  (2009-04-29)
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