Assembly of telomerase RNP

Stable Identifier
R-GGA-417114
Type
Reaction [binding]
Species
Gallus gallus
Compartment
Synonyms
TERC RNA + 2 TERT + 2 DKC1 (dyskerin) => telomerase RNP
ReviewStatus
5/5
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The telomerase RNP complex is formed in the nucleoplasm from TERT protein, TERC RNA, and DKC1 (dyskerin) protein. The complex has been identified and its activity demonstrated in chicken cells (Hrdlickova et al. 2006). Its stoichiometry and the presence of DKC1 as a component of the complex are inferred from studies of purified human telomerase RNP (Cohen et al. 2007).

RUVBL1 (pontin) and RUVBL2 (reptin) are found associated with human telomerase RNPs purified from HeLa cells, and activities of these proteins are required for telomerase RNP assembly in vivo (Venteicher et al. 2008). The exact roles of these proteins in the assembly and function of telomerase RNP in vivo remain unclear, however, so the one protein, RUVBL1 (pontin), for which a chicken ortholog has been identified is annotated simply as positively regulating telomerase RNP formation.

NHP2 (NOLA2) is likewise associated with telomerase ribonucleoprotein complexes (Pogacic et al. 2000) and homozygosity for NHP2 mutations is associated with telomerase failure (dyskeratosis congenital) in humans (Vuillamy et al. 2008). The molecular function of NHP2 remains unclear, however, so its chicken ortholog is annotated simply as positively regulating telomerase RNP formation.

The chicken TERC gene has been identified, based on its content of conserved TERC sequence elements (Chen et al. 2000) and the phenotypes of DT40 cells heterozygous for a null allele of the gene (Faure et al. 2008). Both a full-length the chicken TERT mRNA and numerous shorter mRNAs generated by alternative splicing have been described, but only the full-length form encodes a catalytically active protein (Chang and Delaney 2006; Hrdlickova et al. 2006).

Literature References
PubMed ID Title Journal Year
18358808 Identification of ATPases pontin and reptin as telomerase components essential for holoenzyme assembly

Venteicher, AS, Veenstra, TD, Artandi, SE, Mason, PJ, Meng, Z

Cell 2008
11074001 Human H/ACA small nucleolar RNPs and telomerase share evolutionarily conserved proteins NHP2 and NOP10

Filipowicz, W, Pogacic, V, Dragon, F

Mol Cell Biol 2000
18523010 Mutations in the telomerase component NHP2 cause the premature ageing syndrome dyskeratosis congenita

Kirwan, M, Marrone, A, Walne, A, Digweed, M, Beswick, R, Vulliamy, T, Dokal, I

Proc Natl Acad Sci U S A 2008
16352553 Mechanism of telomerase activation by v-Rel and its contribution to transformation

Hrdlickova, R, Liss, AS, Bose, HR, Nehyba, J

J Virol 2006
17395830 Protein composition of catalytically active human telomerase from immortal cells

Reddel, RR, Lovrecz, GO, Bache, N, Graham, ME, Cohen, SB, Robinson, PJ

Science 2007
18308646 Ku70 prevents genome instability resulting from heterozygosity of the telomerase RNA component in a vertebrate tumour line

Wenner, T, Takeda, S, Faure, V, Farr, CJ, Cooley, C, Morrison, CG, Bourke, E

DNA Repair (Amst) 2008
16806743 Complicated RNA splicing of chicken telomerase reverse transcriptase revealed by profiling cells both positive and negative for telomerase activity

Chang, H, Delany, ME

Gene 2006
10721988 Secondary structure of vertebrate telomerase RNA

Greider, CW, Chen, JL, Blasco, MA

Cell 2000
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