Translesion synthesis by Pol eta

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R-GGA-353382
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Gallus gallus
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Chicken B lymphocyte precursors and DT40 cells diversify their immunoglobulin variable (IgV) genes through homologous recombination (HR) mediated Ig gene conversion. DNA polymerase eta (Pol eta, POLH) is an error prone translesion DNA polymerase that can bypass UV damage induced lesions and that is also involved in IgV hypermutation. Pol eta-deficient chicken cells exhibit hypersensitivity to UV similar to that seen in human patients with xeroderma pigmentosum due to Pol eta deficiency (XP-V). Remarkably, Pol eta cells showed a significant decrease in the frequency of both Ig gene conversion and double strand break induced HR when compared to wild type cells, with these defects being reversed by complementation with human Pol eta. Current evidence therefore suggests that Pol eta has roles in both HR-mediated DNA repair and translesion DNA synthesis (Kawamoto et al. 2005).

Literature References
PubMed ID Title Journal Year
16337602 Dual roles for DNA polymerase eta in homologous DNA recombination and translesion DNA synthesis

Kawamoto, T, Araki, K, Sonoda, E, Yamashita, YM, Harada, K, Kikuchi, K, Masutani, C, Hanaoka, F, Nozaki, K, Hashimoto, N, Takeda, S

Mol Cell 2005
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