MGMT mediated DNA Damage Reversal

Stable Identifier
R-GGA-353300
Type
Reaction [transition]
Species
Gallus gallus
Compartment
nucleoplasm
ReviewStatus
5/5
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MGMT mediated DNA Damage Reversal
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Reactive cellular catabolites can cause DNA damage by methylation of the O-6-guanine. O-6-methylguanine can pair ambiguously with both C and T, and can cause transition mutations. Active reversal of such damage is achieved by the action of 6-O-methylguanine DNA methyltransferase (MGMT). MGMT removes the methyl group from the guanine, causing MGMT to itself become methylated. Because the enzyme is not regenerated at the end of the reaction, this DNA repair reaction is energetically expensive approach because an entire protein molecule is sacrificed for lesion that is corrected.
Literature References
PubMed ID Title Journal Year
6651830 Enzymatic methylation of chemically alkylated DNA and poly(dG-dC) X poly(dG-dC) in B and Z forms

Pfohl-Leszkowicz, A, Boiteux, S, Laval, J, Keith, G, Dirheimer, G

Biochem Biophys Res Commun 1983
1280487 Influence of suramin on some DNA-directed enzymes and primary cultures of chicken embryo and rat cells

Ignatius, A, Tempel, KH

Anticancer Drugs 1992
1418245 O6-alkylguanine-DNA alkyltransferase in the chick embryo during development

Ignatius, A, Tempel, K

Z Naturforsch [C] 1992
9011148 Formation and persistence of N7- and O6-methyl-guanine in DNA of chick embryo brain cells in ovo following administration of N-nitroso-N-methylurea

Schleifer, S, Tempel, K

Zentralbl Veterinarmed A 1996
Participants
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Output
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as an event of
Catalyst Activity

methylated-DNA-[protein]-cysteine S-methyltransferase activity of MGMT [nucleoplasm]

Physical Entity
MGMT [nucleoplasm] (Gallus gallus)
Activity
methylated-DNA-[protein]-cysteine S-methyltransferase activity (GO:0003908)
Authored
Saxena, A  (2008-07-07)
Reviewed
Borowiec, JA  (2009-04-24)
Created
Saxena, A  (2008-06-12)
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