Reactome | UniProt:Q9UNA4 POLI

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UniProt:Q9UNA4 POLI Show undefined attributes

chain	chain:1-740
checksum	C1A5BF0894E91FDF
comment	FUNCTION Error-prone DNA polymerase specifically involved in DNA repair (PubMed:11013228, PubMed:11387224). Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls (PubMed:11013228, PubMed:11387224, PubMed:14630940, PubMed:15199127, PubMed:20388628). Favors Hoogsteen base-pairing in the active site (PubMed:15254543). Inserts the correct base with high-fidelity opposite an adenosine template (PubMed:15254543). Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine (PubMed:11013228). May play a role in hypermutation of immunoglobulin genes (PubMed:12410315). Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity (PubMed:11251121, PubMed:14630940).CATALYTIC ACTIVITY DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphateCOFACTOR Binds nucleotide much more tightly and catalyzes nucleotide insertion much more efficiently in the presence of Mg(2+) than in the presence of Mn(2+).BIOPHYSICOCHEMICAL PROPERTIES Interacts with POLH (PubMed:12606586). Interacts with REV1 (By similarity). Interacts with ubiquitin (By similarity).INTERACTION Binding to ubiquitin mediates localization to replication forks after UV-induced DNA damage.TISSUE SPECIFICITY Ubiquitous. Highly expressed in testis.DOMAIN The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.DOMAIN Ubiquitin-binding motif 1 and ubiquitin-binding motif 2 regulate POLI protein monoubiquitination and localization to nuclear foci after UV-induced DNA damage.PTM Monoubiquitinated. Protein monoubiquitination prevents POLI binding to ubiquitin via the ubiquitin-binding motif 1 and ubiquitin-binding motif 2.SIMILARITY Belongs to the DNA polymerase type-Y family.SEQUENCE CAUTION Truncated N-terminus.SEQUENCE CAUTION Truncated N-terminus.SEQUENCE CAUTION Truncated N-terminus.SEQUENCE CAUTION Truncated N-terminus.
created	[InstanceEdit:217385] Schmidt, EE, 2008-03-27 06:23:53
crossReference	[DatabaseIdentifier:12165732] PDB:4FS2 [DatabaseIdentifier:12406077] RefSeq:NP_009126.2 [DatabaseIdentifier:12283337] Pharos - Targets:Q9UNA4 [DatabaseIdentifier:12241875] PRO:Q9UNA4 [DatabaseIdentifier:12165746] PDB:2L0F [DatabaseIdentifier:12165742] PDB:2DPI [DatabaseIdentifier:12165744] PDB:9DDR [DatabaseIdentifier:12165745] PDB:2L0G [DatabaseIdentifier:12165747] PDB:1T3N [DatabaseIdentifier:12165751] PDB:4EYI [DatabaseIdentifier:12165741] PDB:3H4B [DatabaseIdentifier:12165750] PDB:4EYH [DatabaseIdentifier:12165749] PDB:3EPI [DatabaseIdentifier:12165743] PDB:3H4D [DatabaseIdentifier:12165748] PDB:3EPG [DatabaseIdentifier:12165738] PDB:5ULW [DatabaseIdentifier:12165722] PDB:9DQT [DatabaseIdentifier:12165740] PDB:2DPJ [DatabaseIdentifier:12165730] PDB:2FLL [DatabaseIdentifier:12165706] PDB:9DRC [DatabaseIdentifier:12165736] PDB:3G6Y [DatabaseIdentifier:12165721] PDB:5KT4 [DatabaseIdentifier:12165739] PDB:5ULX [DatabaseIdentifier:12165708] PDB:2ALZ [DatabaseIdentifier:12160032] PDB:2KHW [DatabaseIdentifier:12165716] PDB:5KT2 [DatabaseIdentifier:12165723] PDB:9DQU [DatabaseIdentifier:12165726] PDB:3H40 [DatabaseIdentifier:12165719] PDB:5KT5 [DatabaseIdentifier:12165737] PDB:3OSN [DatabaseIdentifier:12165707] PDB:9DR9 [DatabaseIdentifier:12165718] PDB:3Q8P [DatabaseIdentifier:12165713] PDB:4EBE [DatabaseIdentifier:12165720] PDB:3Q8Q [DatabaseIdentifier:12165729] PDB:2FLN [DatabaseIdentifier:12165704] PDB:5KT6 [DatabaseIdentifier:12165735] PDB:2FLP [DatabaseIdentifier:11489046] ENSEMBL:ENSG00000101751 [DatabaseIdentifier:11594717] ENSEMBL:ENSP00000462664 [DatabaseIdentifier:11717416] ENSEMBL:ENST00000579534 [DatabaseIdentifier:11785424] GeneCards:POLI [DatabaseIdentifier:11800988] GlyGen:Q9UNA4 [DatabaseIdentifier:11833750] HMDB Protein:HMDBP00969 [DatabaseIdentifier:11838508] HPA:ENSG00000101751-POLI [DatabaseIdentifier:12123017] OpenTargets:ENSG00000101751 [DatabaseIdentifier:12165733] PDB:3G6V [DatabaseIdentifier:12165711] PDB:3GV5 [DatabaseIdentifier:12165705] PDB:9DRB [DatabaseIdentifier:12165714] PDB:5KT3 [DatabaseIdentifier:12165727] PDB:1ZET [DatabaseIdentifier:12165701] PDB:3Q8R [DatabaseIdentifier:12165702] PDB:5KT7 [DatabaseIdentifier:12165712] PDB:4EBD [DatabaseIdentifier:12160079] PDB:2MBB [DatabaseIdentifier:12165715] PDB:3GV7 [DatabaseIdentifier:12165710] PDB:9DR7 [DatabaseIdentifier:12165724] PDB:2KTF [DatabaseIdentifier:12165734] PDB:3G6X [DatabaseIdentifier:12165728] PDB:2KHU [DatabaseIdentifier:12165703] PDB:3Q8S [DatabaseIdentifier:12165731] PDB:4FS1 [DatabaseIdentifier:12165725] PDB:9NJH [DatabaseIdentifier:12165700] PDB:3NGD [DatabaseIdentifier:12165709] PDB:4EBC [DatabaseIdentifier:12165717] PDB:3GV8
databaseName	UniProt
dbId	243434
description	recommendedName: DNA polymerase iota ecNumber evidence="6 9 15 16"2.7.7.7 alternativeName: Eta2 alternativeName: RAD30 homolog B
displayName	UniProt:Q9UNA4 POLI
geneName	POLI RAD30B
identifier	Q9UNA4
isSequenceChanged	false
keyword	3D-structure DNA damage DNA repair DNA replication DNA synthesis DNA-binding DNA-directed DNA polymerase Magnesium Manganese Metal-binding Mutator protein Nucleotidyltransferase Nucleus Proteomics identification Reference proteome Schiff base Transferase Ubl conjugation
modified	[InstanceEdit:9983091] Weiser, Joel, 2026-02-20
moleculeType	Protein
name	POLI
otherIdentifier	11201 11721419_at 11755435_a_at 11758286_s_at 16852433 219317_PM_at 219317_at 238992_PM_at 238992_at 3788834 3788835 3788836 3788837 3788838 3788839 3788840 3788841 3788842 3788843 3788845 3788847 3788849 3788850 3788851 3788852 3788853 3788854 3788855 3788857 3788858 3788860 3788861 3788862 3788863 3788864 3788865 3788868 3788869 43932_at 74219_at 8021275 A_14_P125156 A_23_P306890 GE62743 GO:0003677 GO:0003684 GO:0003824 GO:0003887 GO:0005515 GO:0005634 GO:0005654 GO:0006259 GO:0006260 GO:0006281 GO:0006974 GO:0016607 GO:0016740 GO:0016779 GO:0019985 GO:0034061 GO:0036464 GO:0042276 GO:0043226 GO:0046872 GO:0071897 GO:0140097 HMNXSV003008709 Hs.128076.0.A1_3p_at ILMN_1702218 PH_hs_0025673 TC18000186.hg g6005847_3p_at
physicalEntity	[EntityWithAccessionedSequence:R-HSA-5656133] POLI [nucleoplasm] - Homo sapiens
referenceDatabase	[ReferenceDatabase:2] UniProt
referenceGene	[ReferenceDNASequence:ensembl:ENSG00000101751] ENSEMBL:ENSG00000101751 POLI [ReferenceDNASequence:12747827] UCSC:Q9UNA4 POLI [ReferenceDNASequence:11354299] BioGPS Gene:11201 POLI [ReferenceDNASequence:11401280] COSMIC - genes:POLI POLI [ReferenceDNASequence:11409939] CTD Gene:11201 POLI [ReferenceDNASequence:11438567] dbSNP Gene:11201 POLI [ReferenceDNASequence:11817051] HGNC:9182 POLI [ReferenceDNASequence:11893853] KEGG:hsa:11201 POLI [ReferenceDNASequence:11984088] Monarch:11201 POLI [ReferenceDNASequence:12068181] NCBI Gene:11201 POLI [ReferenceDNASequence:12108577] OMIM:605252 POLI
referenceTranscript	[ReferenceRNASequence:12587774] RefSeq:NM_007195.3 POLI
schemaClass	ReferenceGeneProduct
secondaryIdentifier	POLI_HUMAN Q8N590 Q9H0S1 Q9NYH6
sequenceLength	740
species	[Species:48887] Homo sapiens
stId	uniprot:Q9UNA4
url	http://purl.uniprot.org/uniprot/Q9UNA4

Referrals

(referenceEntity)

[EntityWithAccessionedSequence:R-HSA-5656133] POLI [nucleoplasm]

(interactor)