UniProt:Q9UKF6 CPSF3

chain
  • initiator methionine:1
  • chain:2-684
checksum F8AA24EA6FB78377
comment
  • FUNCTION Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as an mRNA 3'-end-processing endonuclease (PubMed:30507380). Also involved in the histone 3'-end pre-mRNA processing (PubMed:30507380). U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. Required for entering/progressing through S-phase of the cell cycle (PubMed:30507380). Required for the selective processing of microRNAs (miRNAs) during embryonic stem cell differentiation via its interaction with ISY1 (By similarity). Required for the biogenesis of all miRNAs from the pri-miR-17-92 primary transcript except miR-92a (By similarity). Only required for the biogenesis of miR-290 and miR-96 from the pri-miR-290-295 and pri-miR-96-183 primary transcripts, respectively (By similarity).COFACTOR Binds 2 Zn(2+) ions per subunit.SUBUNIT Component of the cleavage and polyadenylation specificity factor (CPSF) complex, composed of CPSF1, CPSF2, CPSF3, CPSF4 and FIP1L1. Interacts with CPSF2, CSTF2 and SYMPK. Interacts with TUT1; the interaction is direct and mediates the recruitment of the CPSF complex on the 3'UTR of pre-mRNAs. Interacts with WDR33. Interacts with ZC3H3 (By similarity).INTERACTION Sumoylated on Lys-462, Lys-465 and Lys-545, preferentially by SUMO3.DISEASE The disease is caused by variants affecting the gene represented in this entry.SIMILARITY Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily.
crossReference
databaseName UniProt
dbId 71992
description
  • recommendedName: Cleavage and polyadenylation specificity factor subunit 3 ecNumber evidence="4 7"3.1.27.- alternativeName: Cleavage and polyadenylation specificity factor 73 kDa subunit shortName: CPSF 73 kDa subunit alternativeName: mRNA 3'-end-processing endonuclease CPSF-73
displayName UniProt:Q9UKF6 CPSF3
geneName
  • CPSF3
  • CPSF73
identifier Q9UKF6
isSequenceChanged false
keyword
  • 3D-structure
  • Acetylation
  • Disease variant
  • Endonuclease
  • Epilepsy
  • Hydrolase
  • Intellectual disability
  • Isopeptide bond
  • Metal-binding
  • mRNA processing
  • Nuclease
  • Nucleus
  • Phosphoprotein
  • Proteomics identification
  • Reference proteome
  • Ribonucleoprotein
  • RNA-binding
  • Ubl conjugation
  • Zinc
modified [InstanceEdit:9963647] Weiser, Joel, 2025-08-15
moleculeType Protein
name
  • CPSF3
otherIdentifier
  • 11717571_a_at
  • 16876881
  • 225082_PM_at
  • 225082_at
  • 228227_at
  • 2468919
  • 2468921
  • 2468922
  • 2468923
  • 2468924
  • 2468926
  • 2468927
  • 2468928
  • 2468930
  • 2468931
  • 2468932
  • 2468933
  • 2468935
  • 2468936
  • 2468937
  • 2468938
  • 2468939
  • 2468940
  • 2468941
  • 2468942
  • 2468944
  • 2468946
  • 2468947
  • 2468950
  • 2468951
  • 2468952
  • 2468954
  • 51692
  • 54643_at
  • 8040142
  • A_14_P113018
  • A_23_P28688
  • GE58700
  • GO:0003723
  • GO:0003824
  • GO:0004518
  • GO:0004519
  • GO:0004521
  • GO:0004534
  • GO:0005515
  • GO:0005634
  • GO:0005654
  • GO:0005847
  • GO:0006396
  • GO:0006397
  • GO:0006398
  • GO:0016071
  • GO:0016787
  • GO:0031124
  • GO:0046872
  • GO:0140098
  • GO:0180010
  • GO:1900087
  • GO:1990904
  • HMNXSV003003870
  • Hs.16251.0.S1_3p_at
  • ILMN_2087528
  • PH_hs_0001502
  • TC02000047.hg
physicalEntity
referenceDatabase [ReferenceDatabase:2] UniProt
referenceGene
referenceTranscript
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • CPSF3_HUMAN
  • O14769
  • Q53RS2
  • Q96F36
sequenceLength 684
species [Species:48887] Homo sapiens
stId uniprot:Q9UKF6
url http://purl.uniprot.org/uniprot/Q9UKF6
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