Reactome | UniProt:Q9NRY4 ARHGAP35

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UniProt:Q9NRY4 ARHGAP35 Show undefined attributes

chain	chain:1-1499
checksum	8CCB493414A7E3E6
comment	FUNCTION Rho GTPase-activating protein (GAP) (PubMed:19673492, PubMed:28894085). Binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity (PubMed:19673492). This binding is inhibited by phosphorylation by PRKCA (PubMed:19673492). Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis (By similarity). Transduces signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP) (By similarity). Transduces SRC-dependent signals from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation (By similarity). Modulates Rho GTPase-dependent F-actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation (By similarity). During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth (By similarity). Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'; this function is however unclear and would need additional experimental evidences (PubMed:1894621).ACTIVITY REGULATION Binding of acidic phospholipids inhibits the Rho GAP activity and promotes the Rac GAP activity.SUBUNIT Interacts with RASA1 (By similarity). Interacts with the general transcription factor GTF2I, the interaction sequesters GTF2I in the cytoplasm (PubMed:19393245).SUBCELLULAR LOCATION In response to integrins and SDC4 and upon phosphorylation by PKC, relocalizes from the cytoplasm to regions of plasma membrane ruffling where it colocalizes with polymerized actin.TISSUE SPECIFICITY Detected in neutrophils (at protein level).DOMAIN N-terminal part (1-266) has GTPase activity. Required for proper cellular localization.DOMAIN The pG1 pseudoGTPase domain does not bind GTP.PTM Phosphorylation of Tyr-1105 by PTK6 promotes the association with RASA1, inactivating RHOA while activating RAS. Phosphorylation at Tyr-308 by PDGFRA inhibits binding to GTF2I (PubMed:18829532, PubMed:19393245). Phosphorylated by PRKCA at Ser-1221 and Thr-1226, induces relocalization from the cytoplasm to regions of plasma membrane ruffling and prevents the binding and substrate specificity regulation by phospholipids (PubMed:19673492). In brain, phosphorylated by FYN and SRC (By similarity). During focal adhesion formation, phosphorylated by MAPK1 and MAPK3 at the C-terminal region, probably at Ser-1451, Ser-1476, Thr-1480 and Ser-1483. Phosphorylation by MAPK1 and MAPK3 inhibits GAP function and localizes ARGHAP35 away from newly forming focal adhesions and stress fibers in cells spreading on fibronectin (By similarity). Phosphorylation at Ser-1476 and Thr-1480 by GSK3B requires priming by MAPK and inhibits RhoGAP activity and modulates polarized cell migration (By similarity).SEQUENCE CAUTION Unlikely isoform. Aberrant splice sites.
created	[InstanceEdit:195085] Gopinathrao, G, 2007-04-02 18:48:36
crossReference	[DatabaseIdentifier:12391222] RefSeq:NP_004482.4 [DatabaseIdentifier:12293296] Pharos - Targets:Q9NRY4 [DatabaseIdentifier:12276418] PRO:Q9NRY4 [DatabaseIdentifier:12229099] PDB:8DGQ [DatabaseIdentifier:12229097] PDB:3C5H [DatabaseIdentifier:12229095] PDB:2K85 [DatabaseIdentifier:12229094] PDB:6WAY [DatabaseIdentifier:12229096] PDB:3FK2 [DatabaseIdentifier:12229098] PDB:6PXC [DatabaseIdentifier:12133127] OpenTargets:ENSG00000160007 [DatabaseIdentifier:11848438] HPA:ENSG00000160007-ARHGAP35 [DatabaseIdentifier:11795471] GeneCards:ARHGAP35 [DatabaseIdentifier:11707529] ENSEMBL:ENST00000672722 [DatabaseIdentifier:11584830] ENSEMBL:ENSP00000500409 [DatabaseIdentifier:11483033] ENSEMBL:ENSG00000160007
databaseName	UniProt
dbId	195082
description	recommendedName: fullName evidence="19"Rho GTPase-activating protein 35 alternativeName: Glucocorticoid receptor DNA-binding factor 1 alternativeName: Glucocorticoid receptor repression factor 1 shortName: GRF-1 alternativeName: Rho GAP p190A shortName evidence="13"p190-A
displayName	UniProt:Q9NRY4 ARHGAP35
geneName	ARHGAP35 GRF1 GRLF1 KIAA1722 P190A p190ARHOGAP
identifier	Q9NRY4
isSequenceChanged	false
keyword	3D-structure Cell membrane Cell projection Cytoplasm Cytoskeleton DNA-binding GTP-binding GTPase activation Lipid-binding Membrane Nucleotide-binding Nucleus Phosphoprotein Proteomics identification Reference proteome Repeat Repressor Transcription Transcription regulation
modified	[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
moleculeType	Protein
name	ARHGAP35
otherIdentifier	11725420_at 11725421_a_at 11725422_s_at 11746448_a_at 11748374_a_at 1565881_3p_at 1565881_PM_at 1565881_at 16863521 16863534 202044_PM_at 202044_at 202045_PM_s_at 202045_s_at 202046_PM_s_at 202046_s_at 229394_3p_s_at 229394_PM_s_at 229394_s_at 2909 34724_at 34725_at 3836980 3837002 3837003 3837004 3837005 3837006 3837007 3837011 3837017 3837018 3837019 3837034 3837035 3837036 3837042 3837043 3837044 3837045 3837046 3837047 3837048 3837049 3837050 3837051 3837052 3837053 3837054 3837055 3837056 3837057 3837058 3837059 3837060 3837061 3837062 3837063 3837064 3837069 3837071 3837073 3837075 53559_at 8029856 A_14_P128858 A_23_P39137 A_24_P36299 A_24_P44845 A_33_P3367481 GE58056 GO:0000166 GO:0001843 GO:0003013 GO:0003677 GO:0003924 GO:0005096 GO:0005525 GO:0005543 GO:0005634 GO:0005737 GO:0005815 GO:0005829 GO:0005856 GO:0005886 GO:0007010 GO:0007163 GO:0007165 GO:0007266 GO:0007411 GO:0007413 GO:0008064 GO:0008289 GO:0008360 GO:0008361 GO:0010976 GO:0015629 GO:0016020 GO:0016477 GO:0016787 GO:0021955 GO:0030879 GO:0030900 GO:0030950 GO:0032956 GO:0035024 GO:0036064 GO:0042995 GO:0043010 GO:0043116 GO:0044319 GO:0045724 GO:0048856 GO:0048870 GO:0050770 GO:0051056 GO:0097485 GO:0098772 HMNXSV003049975 Hs.102548.0.A2_3p_at Hs.102548.0.A3_3p_a_at Hs.102548.2.A1_3p_a_at Hs.102548.2.A1_3p_at Hs2.378706.1.S1_3p_at Hs2.378706.1.S1_3p_x_at ILMN_1658145 ILMN_1687362 ILMN_1696845 M73077_at PH_hs_0005686 TC19000669.hg TC19000670.hg g4758481_3p_a_at
physicalEntity	[EntityWithAccessionedSequence:R-HSA-8849056] p-Y1105-ARHGAP35 [cytosol] - Homo sapiens [EntityWithAccessionedSequence:R-HSA-195118] ARHGAP35 [cytosol] - Homo sapiens
referenceDatabase	[ReferenceDatabase:2] UniProt
referenceGene	[ReferenceDNASequence:12694517] UCSC:Q9NRY4 ARHGAP35 [ReferenceDNASequence:12117422] OMIM:605277 ARHGAP35 [ReferenceDNASequence:12062336] NCBI Gene:2909 ARHGAP35 [ReferenceDNASequence:11978243] Monarch:2909 ARHGAP35 [ReferenceDNASequence:11888423] KEGG:hsa:2909 ARHGAP35 [ReferenceDNASequence:11827173] HGNC:4591 ARHGAP35 [ReferenceDNASequence:11429290] dbSNP Gene:2909 ARHGAP35 [ReferenceDNASequence:11424438] CTD Gene:2909 ARHGAP35 [ReferenceDNASequence:11393344] COSMIC - genes:ARHGAP35 ARHGAP35 [ReferenceDNASequence:11348454] BioGPS Gene:2909 ARHGAP35 [ReferenceDNASequence:ensembl:ENSG00000160007] ENSEMBL:ENSG00000160007 ARHGAP35
referenceTranscript	[ReferenceRNASequence:12572969] RefSeq:NM_004491.4 ARHGAP35
schemaClass	ReferenceGeneProduct
secondaryIdentifier	RHG35_HUMAN A7E2A4 Q14452 Q9C0E1
sequenceLength	1499
species	[Species:48887] Homo sapiens
stId	uniprot:Q9NRY4
url	http://purl.uniprot.org/uniprot/Q9NRY4

Referrals

(interactor)	[UndirectedInteraction:11284781] UniProt:Q9NRY4 <-> UniProt:P05120 (IntAct)
(referenceEntity)	[EntityWithAccessionedSequence:R-HSA-8849056] p-Y1105-ARHGAP35 [cytosol] [EntityWithAccessionedSequence:R-HSA-195118] ARHGAP35 [cytosol]
(referenceSequence)	[ModifiedResidue:8849059] O4'-phospho-L-tyrosine at 1105

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