UniProt:Q9H082 RAB33B

chain
  • chain:1-229
checksum 40D8B1A1D4C6CD85
comment
  • FUNCTION The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed:20163571, PubMed:21808068). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed:18448665, PubMed:20163571, PubMed:21808068). RAB33B acts, in coordination with RAB6A, to regulate intra-Golgi retrograde trafficking (PubMed:20163571). Participates in autophagosome formation by recruiting the ATG12-ATG5-ATG16L1 complex to phagophores, probably in a nucleotide-independent manner (PubMed:18448665, PubMed:32960676).CATALYTIC ACTIVITY GTP + H2O = GDP + phosphate + H(+)COFACTOR Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP (Probable). Regulated by GTPase activating proteins (GAPs) such as SGSM2 which increase the GTP hydrolysis activity (PubMed:21808068). Inhibited by GDP dissociation inhibitors (GDIs) (Probable).SUBUNIT Interacts (GTP- and GDP-bound forms) with ATG16L1; the complex consists of a tetramer where two RAB33B molecules bind independently one molecule of the ATG16L1 homodimer; the interaction promotes ATG12-ATG5-ATG16L1 complex recruitment to phagophores (PubMed:18448665, PubMed:21808068, PubMed:32960676). Interacts with ATG16L2; however interaction is approximately hundred times lower than for ATG16L1 (By similarity). Interacts with RIC1 (via C-terminus domain); the interaction is direct with a preference for RAB33B-GTP (PubMed:23091056). Interacts with RGP1 (PubMed:23091056).INTERACTION Under starvation conditions punctate RAB33B-positive structures are often observed in the cytoplasm (By similarity). Under starved conditions RAB33B translocates from the Golgi to phagophores; this translocation is driven by interaction with ATG16L1 (PubMed:32960676).DOMAIN Switch I, switch II and the interswitch regions are characteristic of Rab GTPases and mediate the interactions with Rab downstream effectors. The switch regions undergo conformational changes upon nucleotide binding which drive interaction with specific sets of effector proteins. Although most effectors only bind GTP-bound Rab, ATG16L1 effector binds both GTP- and GDP-bound RAB33B.PTM Prenylated.DISEASE The disease is caused by variants affecting the gene represented in this entry.SIMILARITY Belongs to the small GTPase superfamily. Rab family.
crossReference
databaseName UniProt
dbId 62604
description
  • recommendedName: Ras-related protein Rab-33B ecNumber evidence="6"3.6.5.2
displayName UniProt:Q9H082 RAB33B
geneName
  • RAB33B
identifier Q9H082
isSequenceChanged false
keyword
  • 3D-structure
  • Autophagy
  • Disease variant
  • Dwarfism
  • Golgi apparatus
  • GTP-binding
  • Hydrolase
  • Lipoprotein
  • Membrane
  • Methylation
  • Nucleotide-binding
  • Prenylation
  • Protein transport
  • Proteomics identification
  • Reference proteome
  • Transport
modified [InstanceEdit:9983091] Weiser, Joel, 2026-02-20
moleculeType Protein
name
  • RAB33B
otherIdentifier
  • 11728455_at
  • 11728456_s_at
  • 16970896
  • 221014_PM_s_at
  • 221014_s_at
  • 2744679
  • 2744680
  • 2744681
  • 2744682
  • 2744683
  • 2744684
  • 2744685
  • 2744686
  • 2744688
  • 2744689
  • 2744691
  • 8097507
  • 83452
  • 85186_r_at
  • A_14_P122473
  • A_23_P9565
  • A_24_P268917
  • GE63377
  • GO:0000045
  • GO:0000139
  • GO:0000166
  • GO:0003924
  • GO:0003925
  • GO:0005515
  • GO:0005525
  • GO:0005768
  • GO:0005794
  • GO:0005796
  • GO:0006891
  • GO:0006914
  • GO:0012505
  • GO:0015031
  • GO:0016020
  • GO:0016192
  • GO:0016787
  • GO:0017157
  • GO:0032482
  • GO:0034045
  • GO:0034067
  • GO:0034497
  • GO:0048705
  • GO:0098772
  • GO:0098793
  • GO:1903358
  • GO:1903434
  • GO:2000156
  • TC04000685.hg
  • TC04000686.hg
  • g13786128_3p_s_at
physicalEntity
referenceDatabase [ReferenceDatabase:2] UniProt
referenceGene
referenceTranscript
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • RB33B_HUMAN
  • B2R987
  • Q4W5B0
sequenceLength 229
species [Species:48887] Homo sapiens
stId uniprot:Q9H082
url http://purl.uniprot.org/uniprot/Q9H082
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