FUNCTION Plasma membrane ferroxidase that mediates the extracellular conversion of ferrous/Fe(2+) iron into its ferric/Fe(3+) form. Couples ferroportin which specifically exports ferrous/Fe(2+) iron from cells to transferrin that only binds and shuttles extracellular ferric/Fe(3+) iron throughout the body (PubMed:22961397, PubMed:37277838). By helping iron transfer from cells to blood mainly contributes to dietary iron absorption by the intestinal epithelium and more generally regulates iron levels in the body (By similarity).CATALYTIC ACTIVITY 4 Fe(2+) + O2 + 4 H(+) = 4 Fe(3+) + 2 H2OCOFACTOR Binds 6 Cu cations per monomer.BIOPHYSICOCHEMICAL PROPERTIES kcat is 18 min(-1) for the oxidation of Fe(2+) (at pH 5.0).SUBUNIT Part of a complex composed of SLC40A1/ferroportin, TF/transferrin and HEPH/hephaestin that transfers iron from cells to transferrin.INTERACTION Expressed by intestinal absorptive cells (at protein level) (PubMed:17486601). Also detected in breast, colon, bone trabecular cells and fibroblasts (PubMed:11932491).SIMILARITY Belongs to the multicopper oxidase family.SEQUENCE CAUTION Extended N-terminus.SEQUENCE CAUTION Extended N-terminus.SEQUENCE CAUTION Extended N-terminus.ONLINE INFORMATION Hephaestin entry
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