Reactome | UniProt:Q99ML9 Rnf111

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UniProt:Q99ML9 Rnf111 Show undefined attributes

chain	chain:1-989
checksum	212E3C37BC70DCB5
comment	FUNCTION E3 ubiquitin-protein ligase required for mesoderm patterning during embryonic development (PubMed:11298452). Acts as an enhancer of the transcriptional responses of the SMAD2/SMAD3 effectors, which are activated downstream of BMP (PubMed:14657019). Acts by mediating ubiquitination and degradation of SMAD inhibitors such as SMAD7, inducing their proteasomal degradation and thereby enhancing the transcriptional activity of TGF-beta and BMP (PubMed:14657019). In addition to enhance transcription of SMAD2/SMAD3 effectors, also regulates their turnover by mediating their ubiquitination and subsequent degradation, coupling their activation with degradation, thereby ensuring that only effectors 'in use' are degraded (By similarity). Activates SMAD3/SMAD4-dependent transcription by triggering signal-induced degradation of SNON isoform of SKIL (By similarity). Associates with UBE2D2 as an E2 enzyme (By similarity). Specifically binds polysumoylated chains via SUMO interaction motifs (SIMs) and mediates ubiquitination of sumoylated substrates (PubMed:23530056). Catalyzes 'Lys-63'-linked ubiquitination of sumoylated XPC in response to UV irradiation, promoting nucleotide excision repair (By similarity). Mediates ubiquitination and degradation of sumoylated PML (PubMed:23530056). The regulation of the BMP-SMAD signaling is however independent of sumoylation and is not dependent of SUMO interaction motifs (SIMs) (PubMed:23530056).CATALYTIC ACTIVITY S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.ACTIVITY REGULATION Binds free ubiquitin non-covalently via its RING-type zinc finger. Ubiquitin-binding leads to enhance the E3 ubiquitin-protein ligase activity by stabilizing the ubiquitin-conjugating enzyme E2 (donor ubiquitin) in the 'closed' conformation and activating ubiquitin transfer.PATHWAY Protein modification; protein ubiquitination.SUBUNIT Monomer (By similarity). Interacts with SMAD6, SMAD7, AXIN1, AXIN2 and SKIL isoform SNON (PubMed:14657019). Interacts with (phosphorylated) SMAD2 and SMAD3 (PubMed:17341133). Part of a complex containing RNF111, AXIN1 and SMAD7 (By similarity). Interacts (via SIM domains) with SUMO1 and SUMO2 (PubMed:23530056).INTERACTION Upon TGF-beta treatment, translocates from nucleus to cytosol.ALTERNATIVE PRODUCTS Ubiquitously expressed.DEVELOPMENTAL STAGE Ubiquitously expressed from ES cells to midgestation.DOMAIN The SUMO interaction motifs (SIMs) mediates the binding to polysumoylated substrate.DOMAIN The RING-type zinc finger mediates the E3 ubiquitin-protein ligase activity and binds directly to free ubiquitin. Non-covalent ubiquitin-binding stabilizes the ubiquitin-conjugating enzyme E2 (donor ubiquitin) in the 'closed' conformation and stimulates ubiquitin transfer.DISRUPTION PHENOTYPE Mice rarely develop beyond 15 somites, have a reduced head, fail to undergo turning and die at midgestation.SIMILARITY Belongs to the Arkadia family.
created	[InstanceEdit:217385] Schmidt, EE, 2008-03-27 06:23:53
crossReference	[DatabaseIdentifier:12333874] RefSeq:NP_001344423.1 [DatabaseIdentifier:12333876] RefSeq:NP_001408497.1 [DatabaseIdentifier:12333875] RefSeq:NP_001408496.1 [DatabaseIdentifier:12333873] RefSeq:NP_291082.1 [DatabaseIdentifier:12250505] PRO:Q99ML9 [DatabaseIdentifier:11930905] MGI:1934919 [DatabaseIdentifier:11850053] IntEnz:2.3.2.27 [DatabaseIdentifier:11807188] GlyGen:Q99ML9 [DatabaseIdentifier:11670894] ENSEMBL:ENSMUST00000113595 [DatabaseIdentifier:11670895] ENSEMBL:ENSMUST00000034739 [DatabaseIdentifier:11670893] ENSEMBL:ENSMUST00000215848 [DatabaseIdentifier:11548196] ENSEMBL:ENSMUSP00000109225 [DatabaseIdentifier:11548195] ENSEMBL:ENSMUSP00000149445 [DatabaseIdentifier:11548194] ENSEMBL:ENSMUSP00000034739 [DatabaseIdentifier:11456870] ENSEMBL:ENSMUSG00000032217
databaseName	UniProt
dbId	246057
description	recommendedName: fullName evidence="12"E3 ubiquitin-protein ligase Arkadia ecNumber evidence="8"2.3.2.27 alternativeName: fullName evidence="13"RING finger protein 111 alternativeName: fullName evidence="11"RING-type E3 ubiquitin transferase Arkadia
displayName	UniProt:Q99ML9 Rnf111
geneName	Rnf111
identifier	Q99ML9
isSequenceChanged	false
keyword	Alternative splicing Cytoplasm Developmental protein DNA damage DNA repair Isopeptide bond Metal-binding Nucleus Reference proteome Transferase Ubl conjugation Ubl conjugation pathway Zinc Zinc-finger
modified	[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
moleculeType	Protein
name	Rnf111
otherIdentifier	10594785 112943_at 1423304_a_at 163220_at 17528597 4425086 4467858 4500064 4500584 4506563 4515786 4591112 4621726 4649366 4671441 4695201 4751412 4763440 4788004 4908393 4920669 4931467 4970098 4991798 5000259 5022846 5028446 5031394 5062894 5074150 5088286 5092392 5094670 5245307 5254773 5263561 5311490 5334720 5472305 5505182 5529323 5539462 5575952 5584319 5595067 93836 A_52_P207151 A_52_P444368 A_55_P2093221 GE41929 GO:0000209 GO:0003824 GO:0004842 GO:0005515 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006281 GO:0006511 GO:0006974 GO:0007389 GO:0008270 GO:0016567 GO:0016605 GO:0016740 GO:0030511 GO:0031398 GO:0032184 GO:0032991 GO:0046332 GO:0046872 GO:0061630 GO:0140096 ILMN_1252738 ILMN_2614080 ILMN_2700945 mMC023088 mMC024657 mMR028725
physicalEntity	[EntityWithAccessionedSequence:R-MMU-975981] RNF111 [cytosol] - Mus musculus [EntityWithAccessionedSequence:R-MMU-2186735] Rnf111 [nucleoplasm] - Mus musculus [EntityWithAccessionedSequence:R-MMU-2186765] RNF111 [nucleoplasm] - Mus musculus
referenceDatabase	[ReferenceDatabase:2] UniProt
referenceGene	[ReferenceDNASequence:12729017] UCSC:Q99ML9 Rnf111 [ReferenceDNASequence:12035550] NCBI Gene:93836 Rnf111 [ReferenceDNASequence:11951457] Monarch:93836 Rnf111 [ReferenceDNASequence:11864401] KEGG:mmu:93836 Rnf111 [ReferenceDNASequence:11321668] BioGPS Gene:93836 Rnf111
referenceTranscript	[ReferenceRNASequence:12515767] RefSeq:NM_033604.3 Rnf111 [ReferenceRNASequence:12515765] RefSeq:NM_001421568.1 Rnf111 [ReferenceRNASequence:12515766] RefSeq:NM_001421567.1 Rnf111 [ReferenceRNASequence:12515768] RefSeq:NM_001357494.2 Rnf111
schemaClass	ReferenceGeneProduct
secondaryIdentifier	RN111_MOUSE Q3UVL7 Q6NSW2 Q7TMR3 Q8C881 Q8CBC0
sequenceLength	989
species	[Species:48892] Mus musculus
stId	uniprot:Q99ML9
url	http://purl.uniprot.org/uniprot/Q99ML9

Referrals

(interactor)	[UndirectedInteraction:11257719] UniProt:O35625 <-> UniProt:Q99ML9 (IntAct) [UndirectedInteraction:11238349] UniProt:O15169 <-> UniProt:Q99ML9 (IntAct)
(referenceEntity)	[EntityWithAccessionedSequence:R-MMU-975981] RNF111 [cytosol] [EntityWithAccessionedSequence:R-MMU-2186735] Rnf111 [nucleoplasm] [EntityWithAccessionedSequence:R-MMU-2186765] RNF111 [nucleoplasm]

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